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Human CD30 / TNFRSF8 Protein, Llama IgG2b Fc Tag, low endotoxin

分子别名(Synonym)

TNFRSF8,CD30,D1S166E,Ki-1

表达区间及表达系统(Source)

Human CD30, Llama IgG2b Fc Tag, low endotoxin (TN8-H5250) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Lys 379 (Accession # NP_001234.2).

Predicted N-terminus: Phe 19

蛋白结构(Molecular Characterization)

Unconjugated(Phe 19 - Lys 379) NP_001234.2

This protein carries a llama IgG2b Fc tag at the C-terminus.

The protein has a calculated MW of 66.3 kDa. The protein migrates as 95-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.01 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human CD30, Llama IgG2b Fc Tag, low endotoxin (Cat. No. TN8-H5250) SDS-PAGE gel

Human CD30, Llama IgG2b Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human CD30, Llama IgG2b Fc Tag, low endotoxin (Cat. No. TN8-H5250) at 0.5 μg/mL (100 μL/well) can bind Anti-CD30 Mab, Human IgG1 with a linear range of 0.1-1.6 ng/mL (Routinely tested).

点击申请Protocol

Biotinylated Human SPR

Immobilized Human CD30 Ligand, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CDL-H525b) at 0.5 μg/mL (100 μL/well) can bind Human CD30, Llama IgG2b Fc Tag, low endotoxin (Cat. No. TN8-H5250) with a linear range of 10-78 ng/mL (QC tested).

点击申请Protocol

Please note that there may be a cross-reaction between anti-human IgG Fc antibodies and llama IgG Fc tag, also between anti-llama IgG Fc antibodies and human IgG Fc tag.

背景(Background)

Human CD30 is also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. TNFRSF-8 is expressed by activated, but not by resting, T and B cells. Also, CD30 is expressed on classical Hodgkin Lymphoma cells together with CD15. CD30 is the receptor for TNFSF8/CD30L. CD30 can interact with TRAF2 and TRAF5, and mediate the signal transduction that leads to the activation of NF-kappa-B. TNFRSF8 may play a role in the regulation of cellular growth and transformation of activated lymphoblasts. TNFRSF8 is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity.

 

前沿进展

货号/价格

TN8-H5250-100ug / ¥3500

TN8-H5250-1mg / ¥21000

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CD30靶点信息
英文全称:Tumor necrosis factor receptor superfamily member 8
中文全称:肿瘤坏死因子受体超家族成员8
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:11详情
最高研发阶段:批准上市
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