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Human Siglec-3 / CD33 Protein, Llama IgG2b Fc Tag, low endotoxin

分子别名(Synonym)

CD33,SIGLEC3,gp67

表达区间及表达系统(Source)

Human Siglec-3, Llama IgG2b Fc Tag, low endotoxin (CD3-H5259) is expressed from human 293 cells (HEK293). It contains AA Asp 18 - His 259 (Accession # AAH28152.1).

Predicted N-terminus: Asp 18

蛋白结构(Molecular Characterization)

Unconjugated(Asp 18 - His 259) AAH28152.1

This protein carries a llama IgG2b Fc tag at the C-terminus.

The protein has a calculated MW of 54.6 kDa. The protein migrates as 64-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.01 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human Siglec-3, Llama IgG2b Fc Tag, low endotoxin (Cat. No. CD3-H5259) SDS-PAGE gel

Human Siglec-3, Llama IgG2b Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human Siglec-3, Llama IgG2b Fc Tag, low endotoxin (Cat. No. CD3-H5259) at 5 μg/mL (100 μL/well) can bind Anti-CD33, Human IgG1 with a linear range of 0.078-1.25 μg/mL (QC tested).

点击申请Protocol

Please note that there may be a cross-reaction between anti-human IgG Fc antibodies and llama IgG Fc tag, also between anti-llama IgG Fc antibodies and human IgG Fc tag.

背景(Background)

Myeloid cell surface antigen CD33 is also known as SIGLEC3, Siglecs (sialic acid binding Ig­like lectins) and GP67, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Human CD33 / Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-­terminal Ig-­like V­-type domain, one Ig­-like C2-­type domains, a transmembrane region and a cytoplasmic tail. CD33 / Siglec-3 usually considered myeloid-specific, but it can also be found on some lymphoid cells. In the immune response, CD33 / Siglec-3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33 / Siglec-3 induces apoptosis in acute myeloid leukemia.

 

前沿进展

货号/价格

CD3-H5259-100ug / ¥3500

CD3-H5259-1mg(500ug x 2) / ¥21000

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Siglec-3靶点信息
英文全称:Myeloid cell surface antigen CD33
中文全称:髓系细胞表面抗原CD33
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:10详情
最高研发阶段:批准上市
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