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 >  Protein>GITR Ligand >GIL-H82F8

Biotinylated Human GITR Ligand / TNFSF18 Protein, Avitag™,Fc Tag

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分子别名(Synonym)

TNFSF18,AITRL,TL6,hGITRL,GITR Ligand

表达区间及表达系统(Source)

Biotinylated Human GITR Ligand, Avitag,Fc Tag (GIL-H82F8) is expressed from human 293 cells (HEK293). It contains AA Gln 50 - Ser 177 (Accession # AAH69319.1 ).

Predicted N-terminus: Gly

Request for sequence

蛋白结构(Molecular Characterization)

GITR Ligand Structure

This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a human IgG1 Fc tag

The protein has a calculated MW of 43.5 kDa. The protein migrates as 45-53 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

GITR Ligand SDS-PAGE

Biotinylated Human GITR Ligand, Avitag,Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

GITR Ligand ELISA

Immobilized Human GITR, Fc Tag (Cat. No. GIR-H5254) at 2 μg/mL (100 μL/well) can bind Biotinylated Human GITR Ligand, Avitag,Fc Tag (Cat. No. GIL-H82F8) with a linear range of 4-63 ng/mL (QC tested).

Protocol

 

背景(Background)

Tumor necrosis factor ligand superfamily member 18 (TNFSF18) is also known as Glucocorticoid - induced TNF-related ligand (GITRL or GITR Ligand), Activation - inducible TNF - related ligand (AITRL), which belongs to the tumor necrosis factor family. TNFSF18 is expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells. TNFSF18 / GITRL is up-regulated after stimulation by bacterial lipopolysaccharides (LPS). TNFSF18 Can function as costimulator and lower the threshold for T-cell activation and T-cell proliferation. TNFSF18 / GITR Ligand is important for interactions between activated T-lymphocytes and endothelial cells. TNFSF18 also mediates activation of NF-kappa-B.

 

前沿进展

 
 
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GITR Ligand靶点信息
英文全称:Tumor necrosis factor ligand superfamily member 18
中文全称:肿瘤坏死因子配体超家族成员18
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