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Human Integrin alpha 4 beta 7 (ITGA4&ITGB7) Heterodimer Protein, His Tag&Tag Free (MALS verified)

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分子别名(Synonym)

Integrin alpha 4 beta 7,ITGA4&ITGB7

表达区间及表达系统(Source)

Human ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free (IT7-H52W4) is expressed from human 293 cells (HEK293). It contains AA Tyr 34 - Thr 977 (ITGA4) & Glu 20 - His 723 (ITGB7) (Accession # P13612-1 (ITGA4) & P26010-1 (ITGB7)).

Predicted N-terminus: Tyr 34 (ITGA4) & Glu 20 (ITGB7)

Request for sequence

蛋白结构(Molecular Characterization)

Integrin alpha 4 beta 7 Structure

Human ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA4 and ITGB7, has a calculated MW of 111.4 kDa (ITGA4) & 83.7 kDa (ITGB7). The protein migrates as 55 kDa, 80 kDa, 100-120 kDa and 130-150 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Integrin alpha 4 beta 7 SDS-PAGE

Human ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS

Integrin alpha 4 beta 7 SEC-MALS

The purity of Human ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT7-H52W4) is more than 85% and the molecular weight of this protein is around 210-250 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Integrin alpha 4 beta 7 ELISA

Immobilized Human ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT7-H52W4) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human ITGA4&ITGB7 Antibody, Human IgG4 with a linear range of 0.1-2 ng/mL (QC tested).

Protocol

Integrin alpha 4 beta 7 ELISA

Immobilized Human ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT7-H52W4) at 1 μg/mL (100 μL/well) can bind Human MAdCAM-1, Fc Tag (Cat. No. MAM-H5253) with a linear range of 0.2-4 ng/mL (Routinely tested).

Protocol

 

背景(Background)

Integrin alpha 4 beta 7 is an integrin family adhesion receptor that shares subunits with alpha 4 beta 1 (VLA4) and the E-Cadherin receptor, alpha E beta 7 . Integrin alpha 4 beta 7, which is critical for homing to intestinal mucosa, is induced during T cell activation in Peyer’s patches or mesenteric lymph nodes.Its expression requires signals from local dendritic and stromal cells, including secreted retinoic acid. The HIV-1 envelope protein gp120 binds to the active form of Integrin alpha 4 beta 7, and this may or may not account for the concentration of HIV-1 virus in the gut-associated lymphoid tissue (GALT). Integrin alpha 4 beta 7 may also be involved in lymphocyte trafficking in acute intestinal graft vs. host disease (GVHD).

 

前沿进展

 
 
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Integrin alpha 4 beta 7靶点信息
英文全称:Integrin alpha-4/beta-7
中文全称:整合素α-4/β-7复合体
种类:Homo sapiens
上市药物数量:4详情
临床药物数量:7详情
最高研发阶段:批准上市
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