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Human CD27 / TNFRSF7 Protein, His Tag (MALS Verified)

分子别名(Synonym)

CD27,TNFRSF7,S152,T14,Tp55

表达区间及表达系统(Source)

Human CD27, His Tag (CD7-H522b) is expressed from human 293 cells (HEK293). It contains AA Thr 21 - Ile 192 (Accession # P26842-1).

Predicted N-terminus: Thr 21

蛋白结构(Molecular Characterization)

Online(Thr 21 - Ile 192) P26842-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 21.1 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human CD27, His Tag (Cat. No. CD7-H522b) SDS-PAGE gel

Human CD27, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Human CD27, His Tag (Cat. No. CD7-H522b) MALS images

The purity of Human CD27, His Tag (Cat. No. CD7-H522b) was more than 85% and the molecular weight of this protein is around 38-48 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human CD27, His Tag (Cat. No. CD7-H522b) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD27 Ligand, Avitag,Fc Tag (Cat. No. TN7-H82F4) with a linear range of 2-31 ng/mL (QC tested).

Protocol

背景(Background)

CD Antigen CD27 is also known as Tumor necrosis factor receptor superfamily member 7 (TNFRSF7), which belongs to TNF-receptor superfamily. CD27 / TNFRSF7 is found in most T-lymphocytes. CD27 / TNFRSF7 is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-κB and MAPK8/JNK. CD27 has been shown to interact with SIVA1, TRAF2 and TRAF3.

 

前沿进展

 
货号/价格

CD7-H522b-100ug / ¥3500

CD7-H522b-1mg (500ug X 2) / ¥23100

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CD27靶点信息
英文全称:CD27 antigen
中文全称:CD27抗原
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:4详情
最高研发阶段:临床二期
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