分子别名(Synonym)
TNFRSF9,4-1BB,CD137,CDw137,ILA
表达区间及表达系统(Source)
Human 4-1BB Protein, Mouse IgG2a Fc Tag (41B-H5256) is expressed from human 293 cells (HEK293). It contains AA Leu 24 - Gln 186 (Accession # Q07011-1 ).
Predicted N-terminus: Leu 24
Request for sequence
蛋白结构(Molecular Characterization)
This protein carries a mouse IgG2a Fc tag at the C-terminus
The protein has a calculated MW of 44.2 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素(Endotoxin)
Less than 0.1 EU per μg by the LAL method.
纯度(Purity)
>95% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳(SDS-PAGE)
Human 4-1BB Protein, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性(Bioactivity)-ELISA
Immobilized Human 4‑1BB Ligand, Fc Tag (Cat. No. 41L-H5257) at 1 μg/mL (100 μL/well) can bind Human 4-1BB Protein, Mouse IgG2a Fc Tag (Cat. No. 41B-H5256) with a linear range of 0.6-5 ng/mL (QC tested).
Protocol
Immobilized Human 4‑1BB Ligand, His Tag (Cat. No. 41L-H5249) at 2 μg/mL (100 μL/well) can bind Human 4-1BB Protein, Mouse IgG2a Fc Tag (Cat. No. 41B-H5256) with a linear range of 0.1-4 ng/mL (Routinely tested).
Protocol
Immobilized Human 4-1BB Protein, Mouse IgG2a Fc Tag (Cat. No. 41B-H5256) at 1 μg/mL (100 μL/well) can bind Anti-Human 4-1BB MAb, Human IgG4 with a linear range of 0.2-6 ng/mL (Routinely tested).
Protocol
活性(Bioactivity)-FACS
Flow Cytometry assay shows that Human 4-1BB Protein, Mouse IgG2a Fc Tag (Cat. No. 41B-H5256) can bind to 293T cells overexpressing Human 4-1BBL. The concentration of 4-1BB used is 0.03 μg/mL (Routinely tested).
Protocol
背景(Background)
4-1BB is also known as CD137, tumor necrosis factor receptor superfamily member 9 (TNFRSF9), induced by lymphocyte activation (ILA), is a co-stimulatory molecule of the tumor necrosis factor (TNF) receptor superfamily. CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, CD137 expression is found on dendritic cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation. The best characterized activity of CD137 is its costimulatory activity for activated T cells. Crosslinking of CD137 enhances T cell proliferation, IL-2 secretion survival and cytolytic activity. Further, it can enhance immune activity to eliminate tumors in mice. CD137 can enhance activation-induced T cell apoptosis when triggered by engagement of the TCR/CD3 complex. In addition, 4-1BB/4-1BBL co-stimulatory pathway has been shown to augment secondary CTL responses to several viruses, and meanwhile augment anti-tumor immunity. 4-1BB thus is a promising candidate for immunotherapy of human cancer. CD137 has been shown to interact with TRAF2.