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Biotinylated Human CD155 / PVR Protein, His Tag, Avi Tag (Avitag™)

分子别名(Synonym)

PVR,FLJ25946,PVS,CD155,TAGE4,HVED,NECL5

表达区间及表达系统(Source)

Biotinylated Human CD155, His Tag, Avi Tag (CD5-H82E3) is expressed from human 293 cells (HEK293). It contains AA Trp 21 - Asn 343 (Accession # NP_006496.4).

Predicted N-terminus: Trp 21

蛋白结构(Molecular Characterization)

Precision Avi(Trp 21 - Asn 343) NP_006496.4

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

The protein has a calculated MW of 38.8 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human CD155, His Tag, Avi Tag (Cat. No. CD5-H82E3) SDS-PAGE gel

Biotinylated Human CD155, His Tag, Avi Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human TIGIT, mouse IgG2a Fc tag, low endotoxin (Cat. No. TIT-H5253) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD155, His Tag, Avi Tag (Cat. No. CD5-H82E3) with a linear range of 0.078-1.25 μg/mL (QC tested).

点击申请Protocol

Biotinylated Human SPR

Immobilized Biotinylated Human CD155, His Tag, Avi Tag (Cat. No. CD5-H82E3) at 5 μg/mL (100 μL/well) via streptavidin can bind Human TIGIT Protein, Fc Tag (Cat. No. TIT-H5254) with a linear range of 0.1-2 ng/mL (Routinely tested).

点击申请Protocol

Biotinylated Human ELISA

Immobilized Biotinylated Human CD155, His Tag, Avi Tag (Cat. No. CD5-H82E3) at 5 μg/mL (100 μL/well) via streptavidin can bind Human DNAM-1, Fc Tag (Cat. No. DN1-H5257) with a linear range of 0.1-1 ng/mL (Routinely tested).

点击申请Protocol

背景(Background)

CD155 (cluster of differentiation 155) also known as the poliovirus receptor is a protein that is encoded by the PVR gene. CD155 is a Type I transmembrane glycoprotein in the immunoglobulin superfamily. Commonly known as Poliovirus Receptor (PVR) due to its involvement in the cellular poliovirus infection in primates, CD155's normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells. The role of CD155 in the immune system is unclear, though it may be involved in intestinal humoral immune responses. Subsequent data has also suggested that CD155 may also be used to positively select MHC-independent T cells in the thymus.

 

专利使用说明(Limited Use License)

The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

 

前沿进展

货号/价格

CD5-H82E3-25ug / ¥3500

CD5-H82E3-200ug / ¥13800

文档
联系电话:
400-682-2521(全国)
010-57737274(北京)
021-31604788(上海)
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
techsupport@acrobiosystems.com

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