搜索
 >  其他蛋白>Biotinylated Human TSLP, His Tag, Avi Tag (Avitag™)

Biotinylated Human TSLP, His Tag, Avi Tag (Avitag™)

分子别名(Synonym)

TSLP

表达区间及表达系统(Source)

Biotinylated Human TSLP, His Tag, Avi Tag (TSP-H82Eb) is expressed from human 293 cells (HEK293). It contains AA Tyr 29 - Gln 159 (Accession # Q969D9-1).

Predicted N-terminus: Tyr 29

蛋白结构(Molecular Characterization)

TSLP(Tyr 29 - Gln 159) Q969D9-1

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

The protein has a calculated MW of 18.6 kDa. As a result of glycosylation, the protein migrates as 11 kDa and 18-21 kDa under reducing (R) condition, and 22-28 kDa under non-reducing (NR) condition (SDS-PAGE).

生物素标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

生物素-蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>85% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human TSLP, His Tag, Avi Tag (Cat. No. TSP-H82Eb) SDS-PAGE gel

Biotinylated Human TSLP, His Tag, Avi Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.

 

活性(Bioactivity)

Biotinylated Human TSLP, His Tag, Avi Tag (Catalog # TSP-H82Eb) ELISA bioactivity

Immobilized Human TSLP R, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human TSLP, His Tag, Avi Tag (Cat. No. TSP-H82Eb) with a linear range of 4-31 ng/mL (QC tested).

点击申请Protocol

Biotinylated Human TSLP, His Tag, Avi Tag (Cat. No. TSP-H82Eb) ELISA bioactivity

Immobilized Anti-TSLP, Human IgG2 at 2 μg/mL (100 μL/well) can bind Biotinylated Human TSLP, His Tag, Avi Tag (Cat. No. TSP-H82Eb) with a linear range of 0.1-2 ng/mL (Routinely tested).

点击申请Protocol

 

背景(Background)

Thymic stromal lymphopoietin (TSLP) is an epithelial cell-derived cytokine involved in the pathology of inflammatory skin diseases, and is widely expressed by epithelial cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence (4, 5). Human TSLP has been shown to developing nondeletional central tolerance, amplifying epithelium-induced class switching, inducing atopic diseases and maintaining intestinal noninflammatory environment. Among diverse cells responding to Human TSLP, CD11c+ dendritic cells are the most obviously characterized target cells.

参考文献(References)

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


专利使用说明(Limited Use License)

The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

货号/价格

TSP-H82Eb-25ug / ¥3500

TSP-H82Eb-200ug / ¥13800

文档
联系电话:
400-682-2521(全国)
010-57737274(北京)
021-31604788(上海)
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
techsupport@acrobiosystems.com
问题&评论

×

消息提示

请输入您的联系方式,再点击提交!

确定