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Mouse SIRP alpha / CD172a Protein, Mouse IgG2a Fc Tag, low endotoxin

分子别名(Synonym)

SHPS1,SIRPA,CD172A,BIT,MFR,MYD1,P84,PTPNS1

表达区间及表达系统(Source)

Mouse SIRP alpha, Mouse IgG2a Fc Tag (SIA-M5252) is expressed from human 293 cells (HEK293). It contains AA Lys 32 - Gln 373 (Accession # P97797-1(G365D)).

Predicted N-terminus: Lys 32

蛋白结构(Molecular Characterization)

SIRP alpha(Lys 32 - Gln 373) P97797-1(G365D)

This protein carries a mouse IgG2a Fc tag at the C-terminus.

The protein has a calculated MW of 65.0 kDa. The protein migrates as 90-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Mouse SIRP alpha, Mouse IgG2a Fc Tag (Cat. No. SIA-M5252) SDS-PAGE gel

Mouse SIRP alpha, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)

Mouse SIRP alpha, Mouse IgG2a Fc Tag (Catalog # SIA-M5252) ELISA bioactivity

Immobilized Mouse SIRP alpha, Mouse IgG2a Fc Tag (Cat. No. SIA-M5252) at 2 μg/mL (100 μL/well) can bind Mouse CD47, Fc Tag (Cat. No. CD7-M5251) with a linear range of 8-63 ng/mL (QC tested).

点击申请Protocol

Mouse SIRP alpha, Mouse IgG2a Fc Tag (Cat. No. SIA-M5252) ELISA bioactivity

Immobilized Human CD47 at 10 μg/mL (100 μL/well) can bind Mouse SIRP alpha, Mouse IgG2a Fc Tag (Cat. No. SIA-M5252) with a linear range of 20-313 ng/mL (Routinely tested).

点击申请Protocol

 

背景(Background)

Tyrosine-protein phosphatase non-receptor type substrate 1 (SHPS1) is also known as CD172 antigen-like family member A (CD172a), Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha (SIRPA or SIRP alpha) or p84, is a member of the SIRP family, and also belongs to the immunoglobulin superfamily. SIRP alpha is Ubiquitous and highly expressed in brain. SIRPA / CD172a is immunoglobulin-like cell surface receptor for CD47 and acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA / SHPS-1 supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment and may play a key role in intracellular signaling during synaptogenesis and in synaptic function By similarity. SIRPA / MyD1 involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin and mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.

参考文献(References)

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


货号/价格

SIA-M5252-100ug / ¥2900

SIA-M5252-1mg / ¥19800

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