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Mouse Neuropilin-1 / NRP1 / CD304 Protein, Mouse IgG2a Fc Tag, low endotoxin

分子别名(Synonym)

NRP1,Neuropilin-1,NRP,VEGF165R,CD304

表达区间及表达系统(Source)

Mouse NRP1, Mouse IgG2a Fc Tag (NR1-M5254) is expressed from human 293 cells (HEK293). It contains AA Phe 22 - Pro 856 (Accession # P97333-1).

Predicted N-terminus: Phe 22

蛋白结构(Molecular Characterization)

Unconjugated(Phe 22 - Pro 856) P97333-1

This protein carries a mouse IgG2a Fc tag at the C-terminus.

The protein has a calculated MW of 120.5 kDa. The protein migrates as 125 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Mouse NRP1, Mouse IgG2a Fc Tag (Cat. No. NR1-M5254) SDS-PAGE gel

Mouse NRP1, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human VEGF165, Tag Free (Cat. No. VE5-H4210) at 2 μg/mL (100 μL/well) can bind Mouse NRP1, Mouse IgG2a Fc Tag (Cat. No. NR1-M5254) with a linear range of 0.5-4 ng/mL (QC tested).

点击申请Protocol

背景(Background)

Neuropilin-1 (NRP1) is also known as Vascular endothelial cell growth factor 165 receptor (VEGF165R), CD antigen CD304, which belongs to the neuropilin family. The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis. The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells.

 

前沿进展

 
货号/价格

NR1-M5254-100ug / ¥3500

NR1-M5254-1mg (200ug X 5) / ¥23100

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Neuropilin-1靶点信息
英文全称:Neuropilin-1
中文全称:神经毡蛋白-1
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:1详情
最高研发阶段:临床一期
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