FcRn,FCGRT & B2M
Cynomolgus / Rhesus macaque FcRn Heterodimer Protein (SPR verified), is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # Q8SPV9-1 (FCGRT) & Q8SPW0-1 (B2M)).
Predicted N-terminus: Ala 24 (FCGRT) & Ile 21 (B2M)
Cynomolgus / Rhesus macaque FcRn Heterodimer Protein (SPR verified), produced by co-expression of FCGRT and B2M, has a calculated MW of 32.3 kDa (FCGRT) and 13.1 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with Strep II-tag at the C-terminus. The reducing (R) protein migrates as 36 kDa (FCGRT) and 14 kDa (B2M) respectively due to glycosylation. Cynomolgus / Rhesus macaque FcRn Heterodimer Protein (SPR verified) sequences are identical.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
- 4-8°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Cynomolgus / Rhesus macaque FcRn Heterodimer Protein (SPR verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Ipilimumab at 10 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque FcRn Heterodimer Protein (SPR verified) (Cat. No. FCM-C5284) with a linear range of 0.04-0.3 μg/mL (QC tested).
Immobilized Cynomolgus / Rhesus macaque FcRn Heterodimer Protein (Cat. No. FCM-C5284 ) on CM5 Chip via anti-His antibody, can bind Herceptin with an affinity constant of 0.403 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Authors: Kenniston JA, et al.
Journal: J Biol Chem 2017
Application: ELISA & SPR
FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.
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