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 >  Protein>Activin A >ACA-H421b

Human Activin A / INHBA Protein, premium grade

分子别名(Synonym)

Activin A,INHBA

表达区间及表达系统(Source)

Human Activin A, premium grade (ACA-H421b) is expressed from human 293 cells (HEK293). It contains AA Gly 311 - Ser 426 (Accession # AAH07858.1 ).

Predicted N-terminus: Gly 311

It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.

Request for sequence

蛋白结构(Molecular Characterization)

Activin A Structure

This protein carries no "tag".

The protein has a calculated MW of 13.0 kDa. The protein migrates as 14-15 kDa under reducing (R) condition, and 23-25 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

无菌(Sterility)

The sterility testing was performed by membrane filtration method.

支原体(Mycoplasma)

Negative.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 0.056% TFA in 30% ACN with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Activin A SDS-PAGE

Human Activin A, premium grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-Bioactivity CELL BASE

Activin A CELL

Human Activin A, premium grade (Cat. No. ACA-H421b) inhibits the proliferation of MPC-11 cells. The specific activity of Human Activin A, premium grade is>5.00*10^2 IU/mg, which is calibrated against WHO Reference Reagent Activin A (Human, Recombinant) NIBSC code: 91/626 (QC tested).

Protocol

 

活性(Bioactivity)-ELISA

Activin A ELISA

Immobilized Human Activin A, premium grade (Cat. No. ACA-H421b) at 5 μg/mL (100 μL/well) can bind Human ACVR2A, Fc Tag (Cat. No. ACA-H5269) with a linear range of 0.4-25 ng/mL (QC tested).

Protocol

Activin A ELISA

Immobilized Human Activin A, premium grade (Cat. No. ACA-H421b) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Activin RIIB Protein, His,Avitag (Cat. No. ACB-H82E3) with a linear range of 0.3-5 ng/mL (Routinely tested).

Protocol

 

活性(Bioactivity)-SPR

Activin A SPR

Human Activin RIIB, His Tag (Cat. No. ACB-H5226) immobilized on CM5 Chip can bind Human Activin A, premium grade (Cat. No. ACA-H421b) with an affinity constant of 0.216 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Protocol

 

背景(Background)

Activin and inhibin are two closely related protein complexes that have almost directly opposite biological effects. Activin enhances FSH biosynthesis and secretion, and participates in the regulation of the menstrual cycle. Many other functions have been found to be exerted by activin, including roles in cell proliferation, differentiation, apoptosis, metabolism, homeostasis, immune response, wound repair, and endocrine function. Conversely inhibin down regulates FSH synthesis and inhibits FSH secretion.Activins are nonglycosylated homodimers or heterodimers of various β subunits (βA, βB, βC, and βE in mammals), while Inhibins are heterodimers of a unique α subunit and one of the β subunits. Activin A is a widely expressed homodimer of two βA chains. The βA subunit can also heterodimerize with a βB or βC subunit to form Activin AB and Activin AC, respectively. The 14 kDa mature human βA chain shares 100% amino acid sequence identity with bovine, feline, mouse, porcine, and rat βA.

文献引用(Citations)

 

前沿进展

 
 
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Activin A靶点信息
英文全称:Activin receptor type-1
中文全称:激活素受体-1
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:5详情
最高研发阶段:批准上市
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