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Human B7-H5 / Gi24 / VISTA Protein, Mouse IgG2a Fc Tag, low endotoxin

分子别名(Synonym)

B7-H5,SISP1,Gi24,VISTA

表达区间及表达系统(Source)

Human B7-H5, Mouse IgG2a Fc Tag, low endotoxin (B75-H5258) is expressed from human 293 cells (HEK293). It contains AA Phe 33 - Ala 194 (Accession # AAH20568.1).

Predicted N-terminus: Phe 33

蛋白结构(Molecular Characterization)

Online(Phe 33 - Ala 194) AAH20568.1

This protein carries a mouse IgG2a Fc tag at the C-terminus.

The protein has a calculated MW of 45.0 kDa. The protein migrates as 55-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human B7-H5, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. B75-H5258) SDS-PAGE gel

Human B7-H5, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Biotinylated Human VSIG3, Fc,Avitag (Cat. No. VS3-H82F9) at 5 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Human B7-H5, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. B75-H5258) with a linear range of 0.039-0.625 μg/mL (QC tested).

Protocol

活性(Bioactivity)-SPR

Biotinylated Human SPR

Human B7-H5, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. B75-H5258) Captured on CM5 chip via anti-mouse antibodies surface can bind Human VSIG3, Fc Tag (Cat. No. VS3-H5258) with an affinity constant of 8.87 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Biotinylated Human VSIG3, Fc,Avitag (Cat. No. VS3-H82F9) on SA Biosensor, can bind Human B7-H5, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. B75-H5258) with an affinity constant of 2.6 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Platelet receptor Gi24, also known as B7-H5 and stress-induced secreted protein-1 (Sisp-1), is a protein that in humans is encoded by the C10orf54 gene, which contains 1 Ig-like (immunoglobulin-like) domain. As for C10orf54 gene, C10orf54 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state on ESCs. Human C10orf54 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment.

 

前沿进展

 
货号/价格

B75-H5258-100ug / ¥3000

B75-H5258-1mg (500ug X 2) / ¥19800

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