搜索
 >  免疫检查点蛋白>LILRA5 >LI5-H82E1

Biotinylated Human LILRA5 / CD85f / ILT11 Protein, His,Avitag™

分子别名(Synonym)

LILRA5,CD85f,ILT11,LIR9,LILRB7

表达区间及表达系统(Source)

Biotinylated Human LILRA5, His,Avitag (LI5-H82E1) is expressed from human 293 cells (HEK293). It contains AA Gly 42 - Arg 268 (Accession # A6NI73-1).

Predicted N-terminus: Gly 42

蛋白结构(Molecular Characterization)

Online(Gly 42 - Arg 268) A6NI73-1

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

The protein has a calculated MW of 28.9 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human LILRA5, His,Avitag (Cat. No. LI5-H82E1) SDS-PAGE gel

Biotinylated Human LILRA5, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human ANGPTL7, His Tag (Cat. No. AN7-H52H5) at 5 μg/mL (100 μL/well) can bind Biotinylated Human LILRA5, His,Avitag (Cat. No. LI5-H82E1) with a linear range of 1.25-20 μg/mL (QC tested).

Protocol

背景(Background)

LIRs are immunoglobulin-like receptors that have activating and inhibitory functions in leukocytes. Leukocyte immunoglobulin-like receptor A5 (LILRA5) belongs to a family of receptors known to regulate leukocyte activation. LILRA5, expressed as a membrane-bound receptor and as a secreted molecule. The transmembrane LILRA5 contain a short cytoplasmic domain and a charged arginine residue within the transmembrane region that is likely to mediate its association with another coreceptor. LILRA5 is mostly expressed in myeloid cells, including monocytes and neutrophils.

 

前沿进展

 
货号/价格

LI5-H82E1-25ug / ¥3500

LI5-H82E1-200ug / ¥13800

开发建议
文档
联系电话:
400-682-2521(全国)
010-57737274(北京)
021-26136428(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
techsupport@acrobiosystems.com

消息提示

请输入您的联系方式,再点击提交!

确定