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Biotinylated Human Fc gamma RI / CD64 Protein, His,Avitag™ (SPR & BLI & HPLC verified)

分子别名(Synonym)

FCGR1A,FCG1,FCGR1,IGFR1,CD64,CD64A,FCRI

表达区间及表达系统(Source)

Biotinylated Human CD64, His,Avitag (FCA-H82E8) is expressed from human 293 cells (HEK293). It contains AA Gln 16 - Pro 288 (Accession # P12314-1).

Predicted N-terminus: Gln 16

蛋白结构(Molecular Characterization)

Online(Gln 16 - Pro 288) P12314-1

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

The protein has a calculated MW of 34.2 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by reduced SDS-PAGE.

>90% as determined by SEC-HPLC.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human CD64, His,Avitag (Cat. No. FCA-H82E8) SDS-PAGE gel

Biotinylated Human CD64, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC

Biotinylated Human CD64, His,Avitag (Cat. No. FCA-H82E8) HPLC images

The purity of Biotinylated Human CD64, His,Avitag (Cat. No. FCA-H82E8) was greater than 90% as determined by SEC-HPLC.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Ipilimumab at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD64, His,Avitag (Cat. No. FCA-H82E8) with a linear range of 0.1-3 ng/mL (QC tested).

Protocol

活性(Bioactivity)-SPR

Biotinylated Human SPR

Biotinylated Human CD64, His,Avitag (Cat. No. FCA-H82E8) captured on Biotin CAP - Series S sensor Chip can bind Herceptin® with an affinity constant of 1.66 nM as determined in a SPR assay (Biacore T200) (QC tested).

Protocol

Biotinylated Human SPR

Report

 

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Biotinylated Human CD64, His,Avitag (Cat. No. FCA-H82E8) on SA Biosensor, can bind Herceptin with an affinity constant of 0.515 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Receptors that recognize the Fc portion of IgG are divided into three groups designated Fc gamma RI, RII, and RIII, also known respectively as CD64, CD32, and CD16. Fc gamma RI binds IgG with high affinity and functions during early immune responses. Fc gamma RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. High affinity immunoglobulin gamma Fc receptor I is also known as FCGR1A, FCG1, FCGR1, CD64 and IGFR1, is a type of integral membrane glycoprotein that binds monomeric IgG-type antibodies with high affinity, which belongs to the immunoglobulin superfamily or FCGR1 family. FCGR1A / CD64 contains 3 Ig-like C2-type (immunoglobulin-like) domains. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells.

 

前沿进展

 
货号/价格

FCA-H82E8-25ug / ¥3500

FCA-H82E8-200ug / ¥13800

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