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Human Mesothelin / MSLN (296-580) Protein, Fc Tag, premium grade

分子别名(Synonym)

MSLN,Mesothelin,MPF

表达区间及表达系统(Source)

Human Mesothelin (296-580), Fc Tag, premium grade (MSN-H5253) is expressed from human 293 cells (HEK293). It contains AA Glu 296 - Gly 580 (Accession # AAH09272.1 ).

Predicted N-terminus: Glu 296

It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.

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蛋白结构(Molecular Characterization)

Mesothelin Structure

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 58.6 kDa. The protein migrates as 66-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.01 EU per μg by the LAL method.

无菌(Sterility)

The sterility testing was performed by membrane filtration method.

支原体(Mycoplasma)

Negative.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 12 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Mesothelin SDS-PAGE

Human Mesothelin (296-580), Fc Tag, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Mesothelin SEC-MALS

The purity of Human Mesothelin (296-580), Fc Tag, premium grade (Cat. No. MSN-H5253) is more than 90% and the molecular weight of this protein is around 115-150 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Mesothelin ELISA

Immobilized Human Mesothelin (296-580), Fc Tag, premium grade (Cat. No. MSN-H5253) at 0.5 μg/mL (100 μL/well) can bind Anti-Human MSLN Mab with a linear range of 0.1-3 ng/mL (QC tested).

Protocol

 

CAR阳性表达率检测(Evaluation of CAR expression)

FACS Analysis of Anti-MSLN CAR Expression

Mesothelin CAR_T

293 cells were transfected with anti-MSLN-scFv and RFP tag. 2e5 of the cells were first stained with B. Human Mesothelin (296-580), Fc Tag, premium grade (Cat. No. MSN-H5253, 1 µg/mL) and C. Fc Tag Fusion Protein Control, followed by FITC anti-human IgG Fc. A. Non-transfected 293 cells and C. Fc Tag Fusion Protein Control were used as negative control. RFP was used to evaluate CAR (anti-MSLN-scFv) expression and FITC was used to evaluate the binding activity of Human Mesothelin (296-580), Fc Tag, premium grade (Cat. No. MSN-H5253).

 

背景(Background)

Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.

 

前沿进展

 
 
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Mesothelin靶点信息
英文全称:Mesothelin
中文全称:间皮素
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:33详情
最高研发阶段:临床二期
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