搜索
 >  其他蛋白>Mesothelin >MSN-H5253

Human Mesothelin / MSLN (296-580) Protein, Fc Tag (HPLC verified)

分子别名(Synonym)

MSLN,Mesothelin,MPF

表达区间及表达系统(Source)

Human Mesothelin (296-580), Fc Tag (MSN-H5253) is expressed from human 293 cells (HEK293). It contains AA Glu 296 - Gly 580 (Accession # AAH09272.1).

Predicted N-terminus: Glu 296

蛋白结构(Molecular Characterization)

Online(Glu 296 - Gly 580) AAH09272.1

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 58.6 kDa. The protein migrates as 66-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-HPLC.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 12 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-H5253) SDS-PAGE gel

Human Mesothelin (296-580), Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC

Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-H5253) HPLC images

The purity of Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-H5253) was greater than 90% as determined by SEC-HPLC.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-H5253) at 0.5 μg/mL (100 μL/well) can bind Anti-Human MSLN Mab with a linear range of 0.1-3 ng/mL (QC tested).

Protocol

CAR阳性表达率检测(Evaluation of CAR expression )

FACS Analysis of Anti-MSLN CAR Expression

CAR

293 cells were transfected with anti-MSLN-scFv and RFP tag. 2e5 of the cells were first stained with B. Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-H5253, 1 µg/ml) and C. Fc Tag Fusion Protein Control, followed by FITC anti-human IgG Fc. A. Non-transfected 293 cells and C. Fc Tag Fusion Protein Control were used as negative control. RFP was used to evaluate CAR (anti-MSLN-scFv) expression and FITC was used to evaluate the binding activity of Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-H5253).

Protocol

 

背景(Background)

Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.

 

前沿进展

 
货号/价格

MSN-H5253-100ug / ¥2800

MSN-H5253-1mg (500ug X 2) / ¥18000

开发建议
文档
联系电话:
400-682-2521(全国)
010-57737274(北京)
021-26136428(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
techsupport@acrobiosystems.com
Mesothelin靶点信息
英文全称:Mesothelin
中文全称:间皮素
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:15详情
最高研发阶段:临床二期
查看更多信息

消息提示

请输入您的联系方式,再点击提交!

确定