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Human ANGPTL7 / CDT6 Protein, His Tag

分子别名(Synonym)

ANGPTL7,CDT6

表达区间及表达系统(Source)

Human ANGPTL7, His Tag (AN7-H52H5) is expressed from human 293 cells (HEK293). It contains AA Gln 27 - Pro 346 (Accession # O43827-1).

Predicted N-terminus: Gln 27

蛋白结构(Molecular Characterization)

Online(Gln 27 - Pro 346) O43827-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 39.0 kDa. The protein migrates as 38-40 kDa and 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>85% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human ANGPTL7, His Tag (Cat. No. AN7-H52H5) SDS-PAGE gel

Human ANGPTL7, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.

>获取HPLC数据

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Cynomolgus LILRB4, His Tag (Cat. No. CDK-C5227) at 2 μg/mL (100 μL/well) can bind Human ANGPTL7, His Tag (Cat. No. AN7-H52H5) with a linear range of 8-125 ng/mL (QC tested).

Protocol

活性(Bioactivity)-SPR

Biotinylated Human SPR

Human LILRB4, Fc Tag (SPR verified) (Cat. No. LI4-H5259) captured on CM5 chip via anti-human IgG Fc antibodies surface can bind Human ANGPTL7, His tag (Cat. No. AN7-H52H5) with an affinity constant of 71.6 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

背景(Background)

Angiopoietin-like 7 (ANGPTL7) is also known as Corneal-Derived Transcript 6 (CDT6), and is a secreted glycoprotein that is structurally related to the angiopoietins. It is expressed in the corneal stroma, trabecular meshwork, and sclera and is elevated in glaucoma aqueous humor. Recently, ANGPTL proteins, including ANGPTL-2, -3, -4, -5 and -7, are identified as growth factors in combination with SCF, Thrombopoietin, IGF-II and FGF acidic that enhance the expansion and engraftment of human and mouse hematopoietic stem cells.

 

前沿进展

 
货号/价格

AN7-H52H5-100ug / ¥4100

AN7-H52H5-1mg (200ug X 5) / ¥27000

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