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Human B7-1 / CD80 Protein, Mouse IgG2a Fc Tag, low endotoxin

分子别名(Synonym)

CD80,B7,B7-1,B7.1,BB1,CD28LG,CD28LG1,LAB7

表达区间及表达系统(Source)

Human B7-1, Mouse IgG2a Fc Tag, low endotoxin (B71-H52A4) is expressed from human 293 cells (HEK293). It contains AA Val 35 - Asn 242 (Accession # P33681-1).

Predicted N-terminus: Val 35

蛋白结构(Molecular Characterization)

Online(Val 35 - Asn 242) P33681-1

This protein carries a mouse IgG2a Fc tag at the C-terminus.

The protein has a calculated MW of 50.9 kDa. The protein migrates as 65-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human B7-1, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. B71-H52A4) SDS-PAGE gel

Human B7-1, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human CTLA-4, His Tag (Cat. No. CT4-H5229) at 1 μg/mL (100 μL/well) can bind Human B7-1, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. B71-H52A4) with a linear range of 2-31 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Human B7-1, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. B71-H52A4) at 5 μg/mL (100 μL/well) can bind Human PD-L1, Fc Tag (Cat. No. PD1-H5258) with a linear range of 0.01-0.313 μg/mL (Routinely tested).

Protocol

Biotinylated Human ELISA

Serial dilutions of Ipilimumab were added into Human B7-1, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. B71-H52A4): Biotinylated Human CTLA-4, Fc,Avitag (Cat. No. CT4-H82F3) binding reactions. The half maximal inhibitory concentration (IC50) is 0.8402 μg/mL (Routinely tested).

Protocol

Biotinylated Human ELISA

Immobilized Biotinylated Mouse CD28, His,Avitag (Cat. No. CD8-M82E3) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Human B7-1, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. B71-H52A4) with a linear range of 0.2-5 ng/mL (Routinely tested).

Protocol

活性(Bioactivity)-SPR

Biotinylated Human SPR

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) (Cat. No. CD8-H525a) on CM5 Chip can bind Human B7-1, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. B71-H52A4) with an affinity constant of 2.72 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Human B7-1, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. B71-H52A4) on AMC Biosensor, can bind Human CTLA-4, His Tag (MALS verified) (Cat. No. CT4-H52Hb) with an affinity constant of 1.14 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

Loaded Human B7-1, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. B71-H52A4) on Protein A Biosensor, can bind Human / Cynomolgus / Rhesus macaque CD28, His Tag (Cat. No. CD8-H52Hc) with an affinity constant of 4.6 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

活性(Bioactivity)-FACS

Biotinylated Human FACS

Flow Cytometry assay shows that Human B7-1, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. B71-H52A4) can bind to 293 cell overexpressing human CTLA-4. The concentration of Human B7-1 is 1 μg/mL (Routinely tested).

Protocol

Biotinylated Human FACS

FACS analysis shows that the binding of Human B7-1, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. B71-H52A4) to 293 overexpressing CTLA-4 was inhibited by increasing concentration of neutralizing Anti-human CTLA-4 antibody. The concentration of Human B7-1 is 1 μg /mL. The IC50 is 5.5 μg/mL (Routinely tested).

Protocol

 

背景(Background)

B7-1 and B7-2, together with their receptors CD28 and CTLA­4, constitute one of the dominant co-stimulatory pathways that regulate T­ and B­cell responses. Although both CTLA­4 and CD28 can bind to the same ligands, CTLA­4 binds to B7­1 and B7­2 with a 20 ­ 100 fold higher affinity than CD28 and is involved in the down­regulation of the immune response. B-lymphocyte activation antigen B7-1 (referred to as B7) also known as cluster of Differentiation 80 (CD80), is a member of cell surface immunoglobulin superfamily and is expressed on activated B cells, activated T cells, macrophages and dendritic cells. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. CD80 plays a role in induction of innate immune responses by activating NF-κB-signaling pathway in macrophages. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.

 

前沿进展

 
货号/价格

B71-H52A4-100ug / ¥2600

B71-H52A4-1mg (500ug X 2) / ¥17600

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021-26136428(上海)
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B7-1靶点信息
英文全称:Cluster of Differentiation 80
中文全称:分化群80
种类:Homo sapiens
上市药物数量:2详情
临床药物数量:14详情
最高研发阶段:批准上市
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