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Human Prolactin R / PRLR Protein, Fc Tag

分子别名(Synonym)

Prolactin R,PRLR,PRL-R

表达区间及表达系统(Source)

Human Prolactin R, Fc Tag (PRP-H5251) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - Asp 234 (Accession # P16471-1).

Predicted N-terminus: Gln 25

蛋白结构(Molecular Characterization)

Online(Gln 25 - Asp 234) P16471-1

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 51.0 kDa. The protein migrates as 64 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human Prolactin R, Fc Tag (Cat. No. PRP-H5251) SDS-PAGE gel

Human Prolactin R, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human Prolactin, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. PRN-H5257) at 1 μg/mL (100 μL/well) can bind Human Prolactin R, Fc Tag (Cat. No. PRP-H5251) with a linear range of 2-39 ng/mL (QC tested).

Protocol

Biotinylated Human SPR

Immobilized Human GH, Tag Free at 2 μg/mL (100 μL/well) can bind Human Prolactin R, Fc Tag (Cat. No. PRP-H5251) with a linear range of 0.4-6 ng/mL (Routinely tested).

Protocol

背景(Background)

As a transmembrane receptor, the prolactin receptor (PRL-R) interacts with prolactin. In addition, the PRL-R also binds and is activated by growth hormone (GH) and human placental lactogen (hPL). Preclinical investigations, epidemiological studies and analyses of tissue specimens from patients strongly support the contribution of prolactin receptor (PRLR) signaling to breast and prostate tumorigenesis and cancer progression. Moreover, The PRLR has been found to be essential for lobuloalveolar maturation of the mammary glands during pregnancy, as evidenced by the fact that PRLR knockout mice show severely impaired development of lobuloalveolar structures.

 

前沿进展

 
货号/价格

PRP-H5251-100ug / ¥3500

PRP-H5251-1mg / ¥23100

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