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Biotinylated Human CD4 Protein, His,Avitag™

分子别名(Synonym)

CD4,CD4mut,LEU3

表达区间及表达系统(Source)

Biotinylated Human CD4, His,Avitag (CD4-H82E8) is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Pro 396 (Accession # P01730-1).

Predicted N-terminus: Lys 26

蛋白结构(Molecular Characterization)

Online(Lys 26 - Pro 396) P01730-1

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

The protein has a calculated MW of 45.0 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human CD4, His,Avitag (Cat. No. CD4-H82E8) SDS-PAGE gel

Biotinylated Human CD4, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Ibalizumab at 1 μg/mL (100 μL/well) can bind Biotinylated Human CD4, His,Avitag (Cat. No. CD4-H82E8) with a linear range of 0.2-3 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized HIV-1 [HIV-1/Clade C (16055)] GP120, His Tag (Cat. No. GP5-V15224) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD4, His,Avitag (Cat. No. CD4-H82E8) with a linear range of 1-16 ng/mL (Routinely tested).

Protocol

Biotinylated Human ELISA

Immobilized HIV-1 [HIV-1/Clade E (CM244)] GP120, His Tag (Cat. No. GP4-V15223) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD4, His,Avitag (Cat. No. CD4-H82E8) with a linear range of 2-78 ng/mL (Routinely tested).

Protocol

背景(Background)

T-cell surface glycoprotein CD4 is also known as T-cell surface antigen T4/Leu-3. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is accessory protein for MHC class-II antigen/T-cell receptor interaction. CD4 induces the aggregation of lipid rafts. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Therefore, medical professionals refer to the CD4 count to decide when to begin treatment for HIV-infected patients.

 

前沿进展

 
货号/价格

CD4-H82E8-25ug / ¥3500

CD4-H82E8-200ug / ¥13800

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CD4靶点信息
英文全称:T-cell surface antigen CD4
中文全称:T细胞表面抗原CD4
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:45详情
最高研发阶段:批准上市
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