Integrin alpha E beta 7,ITGAE&ITGB7
Human ITGAE&ITGB7 Heterodimer Protein, His Tag&Tag Free (IT7-H52W7) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Ser 1124 (ITGAE) & Glu 20 - His 723 (ITGB7) (Accession # P38570-1 (ITGAE) & P26010-1 (ITGB7)).
Predicted N-terminus: Phe 19 (ITGAE) & Glu 20 (ITGB7)
Human ITGAE&ITGB7 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAE and ITGB7, has a calculated MW of 128.6 kDa (ITGAE) and 81.8 kDa (ITGB7). Subunit ITGAE is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB7 contains no tag but a basic tail at the C-terminus. The reducing (R) protein migrates as 135-150 kDa (ITGAE) and 100-115 kDa (ITGB7) respectively due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by reduced SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human ITGAE&ITGB7 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Integrin alpha E beta 7 consist of two major subunits: Integrin alpha E (ITGAE) also known as CD103 (cluster of differentiation 103) and Integrin beta-7 is an integrin protein that in humans is encoded by the ITGB7 gene. Integrin alpha E beta 7 (CD103) is expressed mainly by cells of the T lymphocyte lineage within mucosal tissues. This is a strikingly narrow pattern of expression compared with that of other integrins. Lymphocytes expressing alpha E beta 7 are abundant in the gut and comprise a major part of the total T cell complement of the body. The effectiveness of lung transplantation is marred by the relatively high incidence of rejection. The lung normally contains a large population of lymphocytes in contact with the airway epithelium, a proportion of which expresses the mucosal integrin, alpha(E)(CD103)beta(7). This integrin is not a homing receptor, but is thought to retain lymphocytes at the epithelial surface.