膜杰作
Star Staining
分子别名（Synonym）
SLAMF7,CD319,CS1,CRACC,19A,FOAP-12
表达区间及表达系统（Source）
Biotinylated Human SLAMF7, Avitag,His Tag (SL7-H82E0) is expressed from human 293 cells (HEK293). It contains AA Ser 23 - Met 226 (Accession # Q9NQ25-1).
Predicted N-terminus: Ser 23
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag
The protein has a calculated MW of 25.6 kDa. The protein migrates as 40-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Biotinylated Human SLAMF7, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-HPLC
The purity of Biotinylated Human SLAMF7, Avitag,His Tag (Cat. No. SL7-H82E0) was greater than 90% as determined by SEC-HPLC.
活性（Bioactivity）-ELISA
Immobilized Anti-SLAMF7/CS1 Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human SLAMF7, Avitag,His Tag (Cat. No. SL7-H82E0) with a linear range of 0.2-2 ng/mL (QC tested).
Protocol
Immobilized Humanized Anti-Human CS1 MAb at 1 μg/mL (100 μL/well) can bind Biotinylated Human SLAMF7, Avitag,His Tag (Cat. No. SL7-H82E0) with a linear range of 0.6-5 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-FACS
2e5 of Anti-SLAMF7 CAR-293 cells were stained with 100 μL of 0.1 μg/mL of Biotinylated Human SLAMF7, Avitag, His Tag (Cat. No. SL7-H82E0) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (QC tested).
Protocol
背景（Background）
SLAM family member 7 (SLAMF7) is also known as CD2-like receptor-activating cytotoxic cells (CRACC), Membrane protein FOAP-12, CD antigen CD319, Novel Ly9, Protein 19A, which is a single-pass type I membrane protein and a member of the CD2 family of cell surface receptors. SLAMF7 is expressed in spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung and trachea. Isoform 1 of SLAMF7 mediates NK cell activation through a SH2D1A-independent extracellular signal-regulated ERK-mediated pathway. May play a role in lymphocyte adhesion. Isoform 3 of SLAMF7 does not mediate any NK cell activation.
