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 >  Protein>M-CSF R >CSR-H82E0

Biotinylated Human M-CSF R / CSF1R / CD115 Protein, Avitag™,His Tag

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分子别名(Synonym)

CSF1R,C-FMS,CD115,CSFR,FIM2,FMS,M-CSFR

表达区间及表达系统(Source)

Biotinylated Human M-CSF R, Avitag,His Tag (CSR-H82E0) is expressed from human 293 cells (HEK293). It contains AA Ile 20 - Glu 512 (Accession # P07333-1 ).

Predicted N-terminus: Ile 20

Request for sequence

蛋白结构(Molecular Characterization)

M-CSF R Structure

This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag

The protein has a calculated MW of 57.1 kDa. The protein migrates as 65-92 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

M-CSF R SDS-PAGE

Biotinylated Human M-CSF R, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

M-CSF R ELISA

Immobilized Human M-CSF, His Tag (Cat. No. MCF-H5247) at 5 μg/mL (100 μL/well) can bind Biotinylated Human M-CSF R, Avitag,His Tag (Cat. No. CSR-H82E0) with a linear range of 0.01-0.5 μg/mL (QC tested).

Protocol

 

背景(Background)

Colony stimulating factor 1 receptor (CSF1R) is also known as macrophage colony-stimulating factor receptor (M-CSFR), CD115 Cluster of Differentiation 115 (CD115), C-FMS, CSFR, FIM2, FMS, and is a member of the typeⅢ subfamily of receptor tyrosine kinases (RTKs). CSF1R is a receptor for a cytokine called colony stimulating factor 1, The protein encoded by the CSFR1 gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most, if not all, of the biological effects of this cytokine. Ligand binding activates CSFR1 through a process of oligomerization and transphosphorylation . Mutations in CSF1R are associated with chronic myelomonocytic leukemia and type M4 acute myeloblastic leukemia. Increased levels of CSF1R1 are found in microglia in Alzheimer's disease and after brain injuries. The increased receptor expression causes microglia to become more active. Both CSF1R, and its ligand colony stimulating factor 1 play an important role in the development of the mammary gland and may be involved in the process of mammary gland carcinogenesis.

 

前沿进展

 
 
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M-CSF R靶点信息
英文全称:Colony stimulating factor 1 receptor
中文全称:集落刺激因子1受体
种类:Homo sapiens
上市药物数量:8详情
临床药物数量:37详情
最高研发阶段:批准上市
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