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 >  Protein>TRAIL R2 >TR2-H82E6

Biotinylated Human TRAIL R2 / DR5 / TNFRSF10B Protein, Avitag™,His Tag (MALS verified)

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分子别名(Synonym)

TNFRSF10B,TRAILR2,TRAIL-R2,CD262,DR5,KILLER,TRICK2,ZTNFR9,TRICKB

表达区间及表达系统(Source)

Biotinylated Human TRAIL R2, Avitag,His Tag (TR2-H82E6) is expressed from human 293 cells (HEK293). It contains AA Ile 56 - Glu 182 (Accession # O14763-1).

Predicted N-terminus: Ile 56

Request for sequence

蛋白结构(Molecular Characterization)

Online(Ile 56 - Glu 182) O14763-1

This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

The protein has a calculated MW of 16.9 kDa. The protein migrates as 22 kDa and 24 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human TRAIL R2, Avitag,His Tag (Cat. No. TR2-H82E6) SDS-PAGE gel

Biotinylated Human TRAIL R2, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Biotinylated Human TRAIL R2, Avitag,His Tag (Cat. No. TR2-H82E6) MALS images

The purity of Biotinylated Human TRAIL R2, Avitag,His Tag (Cat. No. TR2-H82E6) is more than 90% and the molecular weight of this protein is around 20-30 kDa verified by SEC-MALS.

Report

背景(Background)

Tumor necrosis factor receptor superfamily member 10B (TNFRSF10B) is also known as TNF-related apoptosis-inducing ligand receptor 2 (TRAILR2), Death receptor 5 (DR5), CD262, KILLER, is a member of the TNF-receptor superfamily, and contains an intracellular death domain. TNFRSF10B / DR-5 is widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines. TRAILR2 / CD262 / DR5 is the receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD (a death domain containing adaptor protein) of TRAIL-R2 / TNFRSF10B recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. CD262 / DR5 Promotes the activation of NF-kappa-B. DR5 is essential for ER stress-induced apoptosis and is regulated by p53/TP53.

 

 

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TRAIL R2靶点信息
英文全称:Tumor necrosis factor receptor superfamily member 10B
中文全称:肿瘤坏死因子受体超家族成员10B
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:11详情
最高研发阶段:批准上市
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