分子别名(Synonym)
Basigin,BSG,5F7,CD147,EMMPRIN,M6,OK,TCSF
表达区间及表达系统(Source)
Biotinylated Human EMMPRIN, Avitag,His Tag (CD7-H82E0) is expressed from human 293 cells (HEK293). It contains AA Ala 22 - His 205 (Accession # P35613-2).
Predicted N-terminus: Ala 22
Request for sequence
蛋白结构(Molecular Characterization)
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag
The protein has a calculated MW of 22.8 kDa. The protein migrates as 30-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记(Labeling)
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度(Protein Ratio)
Passed as determined by the HABA assay / binding ELISA.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>92% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳(SDS-PAGE)
Biotinylated Human EMMPRIN, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 92%.
SEC-MALS
The purity of Biotinylated Human EMMPRIN, Avitag,His Tag (Cat. No. CD7-H82E0) is more than 90% and the molecular weight of this protein is around 25-35 kDa verified by SEC-MALS.
Report
活性(Bioactivity)-ELISA
Immobilized Biotinylated Human EMMPRIN, Avitag,His Tag (Cat. No. CD7-H82E0) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-EMMPRIN Antibody, Mouse IgG1 with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
Immobilized Anti-EMMPRIN Antibody, Mouse IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human EMMPRIN, Avitag,His Tag (Cat. No. CD7-H82E0) with a linear range of 0.2-5 ng/mL (Routinely tested).
Protocol
背景(Background)
CD147, also known as Basigin (BSG), or extracellular matrix metalloproteinase inducer (EMMPRIN). The human basigin protein contains 269 amino acids that form two heavily glycosylated C2 type immunoglobulin-like domains at the N-terminal extracellular portion. A second form of basigin has also been characterized that contains one additional immunoglobulin-like domain in its extracellular portion. As members of the immunoglobulin superfamily play fundamental roles in intercellular recognition involved in various immunologic phenomena, differentiation, and development, basigin is thought also to play a role in intercellular recognition and regulate several distinct functions, such as spermatogenesis, expression of the monocarboxylate transporter and the responsiveness of lymphocytes. Basigin is a type I integral membrane receptor that has many ligands, including the cyclophilin (CyP) proteins Cyp-A and CyP-B and certain integrins.