CD3E & CD3D,CD3 delta & CD3 epsilon,CD3 delta / epsilon
Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free (CDD-H52W4) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P07766-1 (CD3E) & P04234-1 (CD3D)).
Predicted N-terminus: Asp 23 (CD3E) & Phe 22 (CD3D)
Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free is produced by co-expression of CD3E and CD3D, has a calculated MW of 16.6 kDa (CD3E) and 13.4 kDa (CD3D). Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3D contains no tag. The reducing (R) heterodimer protein migrates as 24-32 kDa (CD3E) and 22 kDa (CD3D) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free (Cat. No. CDD-H52W4) at 2 μg/mL (100 μL/well) can bind OKT3 (mouse IgG2a) with a linear range of 0.01-0.08 μg/mL (QC tested).
Authors: Ostrand-rosenberg, Suzanne ,et al.
Journal: US20180291114A1 2018
Authors: Horn LA, et al.
Journal: Oncotarget 2017
T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.