分子别名(Synonym)
SHPS1,SIRPA,CD172A,BIT,MFR,MYD1,P84,PTPNS1
表达区间及表达系统(Source)
Biotinylated Human SIRP alpha, Fc,Avitag (CDA-H82F2) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Arg 370 (Accession # P78324-1).
Predicted N-terminus: Glu 31
Request for sequence
蛋白结构(Molecular Characterization)
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 65.8 kDa. The protein migrates as 75-105 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记(Labeling)
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度(Protein Ratio)
Passed as determined by the HABA assay / binding ELISA.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂(Formulation)
Supplied as 0.2 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
运输(Shipping)
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储(Storage)
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
电泳(SDS-PAGE)
Biotinylated Human SIRP alpha, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human SIRP alpha, Fc,Avitag (Cat. No. CDA-H82F2) is more than 90% and the molecular weight of this protein is around 155-170 kDa verified by SEC-MALS.
Report
活性(Bioactivity)-ELISA
Immobilized Human CD47, His Tag (Cat. No. CD7-H5227) at 5 μg/mL (100 μL/well) can bind Biotinylated Human SIRP alpha, Fc,Avitag (Cat. No. CDA-H82F2) with a linear range of 0.05-0.2 μg/mL (QC tested).
Protocol
Serial dilutions of Anti-Human CD47 Neutralizing Antibody were added into Human CD47, Fc Tag (Cat. No. CD7-H5256): Biotinylated Human SIRP alpha, Fc,Avitag (Cat. No. CDA-H82F2) binding reactions. The half maximal inhibitory concentration (IC50) is 0.5431 μg/mL (Routinely tested).
Protocol
活性(Bioactivity)-FACS
FACS assay shows that Biotinylated Human SIRP alpha, Fc,Avitag (Cat. No. CDA-H82F2) can bind to Jurkat cell expressing CD47. The concentration of SIRP alpha used is 3 μg/mL (QC tested).
Protocol
FACS analysis shows that the binding of Biotinylated Human SIRP alpha, Fc,Avitag (Cat. No. CDA-H82F2) to Jurkat expressing CD47 was inhibited by increasing concentration of neutralizing Anti- Human CD47 antibody. The concentration of SIRP alpha used is 1 μg/mL. IC50=0.1303 μg/mL (Routinely tested).
Protocol
背景(Background)
Tyrosine-protein phosphatase non-receptor type substrate 1 (SHPS1) is also known as CD172 antigen-like family member A (CD172a), Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha (SIRPA or SIRP alpha) or p84, is a member of the SIRP family, and also belongs to the immunoglobulin superfamily. SIRP alpha is Ubiquitous and highly expressed in brain. SIRPA / CD172a is immunoglobulin-like cell surface receptor for CD47 and acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA / SHPS-1 supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment and may play a key role in intracellular signaling during synaptogenesis and in synaptic function By similarity. SIRPA / MyD1 involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin and mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.