分子别名（Synonym）
IL-17A,Interleukin-17A,CTLA-8,IL-17
表达区间及表达系统（Source）
Human IL17A, Tag Free (ILA-H5118) is expressed from E.coli cells. It contains AA Gly 24 - Ala 155 (Accession # Q16552).
Predicted N-terminus: Met
蛋白结构（Molecular Characterization）
This protein carries no "tag".
The protein has a calculated MW of 15.3 kDa. the protein migrates as 18 kDa under reducing (R) condition, and 28 kDa under non-reducing (NR) condition (SDS-PAGE).
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human IL17A, Tag Free on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human IL17A, Tag Free (Cat. No. ILA-H5118) was more than 95% and the molecular weight of this protein is around 30-40 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human IL-17A, Tag Free (Cat. No. ILA-H5118) at 0.5 μg/mL (100 μL/well) can bind Human IL-17 RA, His Tag (Cat. No. ILA-H5222) with a linear range of 8-125 ng/mL (QC tested).
Protocol
Immobilized Human IL-17A, Tag Free (Cat. No. ILA-H5118) at 2 μg/mL (100 μL/well) can bind Anti-IL17A MAb, Human IgG1 with a linear range of 0.08-1 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Human IL-17 RA, Fc Tag (Cat. No. ILA-H5257) on Protein A Biosensor, can bind Human IL17A, Tag Free (Cat. No. ILA-H5118) with an affinity constant of 0.21 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
活性（Bioactivity）-Cell based assay
Human IL17/ IL17A Protein, Free Tag(Cat. No. ILA-H5118) stimulates secretion of IL-6 by NIH-3T3 cells. The ED50 for this effect is 1.444-6.941 ng/ml.
Protocol
背景（Background）
Interleukin-17A (IL17A) is also known as cytotoxic T-lymphocyte-associated antigen 8 (CTLA8),which is a proinflammatory cytokine produced by activated T cells. IL17A can regulate the activities of NF-kappaB and mitogen-activated protein kinases. Also,IL17A can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO).Furthermore,IL17A has been found both in glycosylated and nonglycosylated forms. High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
