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Human IL-17A / CTLA-8 Protein, Tag Free (MALS verified)

分子别名(Synonym)

IL-17A,Interleukin-17A,CTLA-8,IL-17

表达区间及表达系统(Source)

Human IL17A, Tag Free (ILA-H5118) is expressed from E.coli cells. It contains AA Gly 24 - Ala 155 (Accession # Q16552).

Predicted N-terminus: Met

蛋白结构(Molecular Characterization)

Online(Gly 24 - Ala 155) Q16552

This protein carries no "tag".

The protein has a calculated MW of 15.3 kDa. the protein migrates as 18 kDa under reducing (R) condition, and 28 kDa under non-reducing (NR) condition (SDS-PAGE).

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human IL17A, Tag Free (Cat. No. ILA-H5118) SDS-PAGE gel

Human IL17A, Tag Free on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Human IL17A, Tag Free (Cat. No. ILA-H5118) MALS images

The purity of Human IL17A, Tag Free (Cat. No. ILA-H5118) was more than 95% and the molecular weight of this protein is around 30-40 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human IL-17A, Tag Free (Cat. No. ILA-H5118) at 0.5 μg/mL (100 μL/well) can bind Human IL-17 RA, His Tag (Cat. No. ILA-H5222) with a linear range of 8-125 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Human IL-17A, Tag Free (Cat. No. ILA-H5118) at 2 μg/mL (100 μL/well) can bind Anti-IL17A MAb, Human IgG1 with a linear range of 0.08-1 ng/mL (Routinely tested).

Protocol

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Human IL-17 RA, Fc Tag (Cat. No. ILA-H5257) on Protein A Biosensor, can bind Human IL17A, Tag Free (Cat. No. ILA-H5118) with an affinity constant of 0.21 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

活性(Bioactivity)-Cell based assay

Biotinylated Human CELL_BASE

Human IL17/ IL17A Protein, Free Tag(Cat. No. ILA-H5118) stimulates secretion of IL-6 by NIH-3T3 cells. The ED50 for this effect is 1.444-6.941 ng/ml.

Protocol

 

背景(Background)

Interleukin-17A (IL17A) is also known as cytotoxic T-lymphocyte-associated antigen 8 (CTLA8),which is a proinflammatory cytokine produced by activated T cells. IL17A can regulate the activities of NF-kappaB and mitogen-activated protein kinases. Also,IL17A can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO).Furthermore,IL17A has been found both in glycosylated and nonglycosylated forms. High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

 

前沿进展

 
货号/价格

ILA-H5118-25ug / ¥1300

ILA-H5118-100ug / ¥3500

ILA-H5118-1mg (250ug X 4) / ¥23100

开发建议
文档
联系电话:
400-682-2521(全国)
010-57737274(北京)
021-26136428(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
techsupport@acrobiosystems.com
IL-17A靶点信息
英文全称:Interleukin 17A
中文全称:白细胞介素-17A
种类:Homo sapiens
上市药物数量:3详情
临床药物数量:16详情
最高研发阶段:批准上市
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