Human Frizzled-2, Fc Tag (FZ2-H5251) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Ser 156 (Accession # AAI13403).
Predicted N-terminus: Gln 24
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 41.7 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
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For long term storage, the product should be stored at lyophilized state at -20°C or lower.
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This product is stable after storage at:
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Human Frizzled-2, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Authors: Tao L, et al.
Journal: Nature 2016
Application: Pulldown assay & BLI assay
Frizzled-2 (FZD2) is also known as FzE2, which belongs to the G-protein coupled receptor Fz/Smo family. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. FZD2 contains one FZ (frizzled) domain. FZD2 may be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. The Lys-Thr-X-X-X-Trp motif of FZD2 interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.