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 >  Protein>CD36 >CD6-H5221

Human CD36 / SR-B3 Protein, His Tag (MALS verified)

分子别名(Synonym)

CD36,SCARB3,BDPLT10,CHDS7,FAT,GP3B,GP4,GPIV,PASIV,Platelet Glycoprotein 4,glycoprotein IV,gpIV,glycoprotein IIIb,gpIIIb

表达区间及表达系统(Source)

Human CD36, His Tag (CD6-H5221) is expressed from human 293 cells (HEK293). It contains AA Gly 30 - Asn 439 (Accession # NP_001001547.1 ).

Predicted N-terminus: Gly 30

Request for sequence

蛋白结构(Molecular Characterization)

CD36 Structure

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 48.5 kDa. The protein migrates as 66-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

CD36 SDS-PAGE

Human CD36, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

CD36 SEC-MALS

The purity of Human CD36, His Tag (Cat. No. CD6-H5221) is more than 90% and the molecular weight of this protein is around 58-78 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

CD36 ELISA

Immobilized Mouse/IgG1,CD36 Antibody at 1 μg/mL (100 μL/well) can bind Human CD36, His Tag (Cat. No. CD6-H5221) with a linear range of 1-16 ng/mL (QC tested).

Protocol

CD36 ELISA

Immobilized Human CD36, His Tag (Cat. No. CD6-H5221) at 1 μg/mL (100 μL/well) can bind Mouse/IgG1,CD36 Antibody with a linear range of 0.1-6 ng/mL (Routinely tested).

Protocol

 

背景(Background)

CD36 (Cluster of Differentiation 36) is also known as platelet membrane glycoprotein IV (GPIV), fatty acid translocase (FAT), thrombospondin receptor, collagen receptor, and scavenger receptor class B, member 3 (SRB3), is a member of the class B scavenger receptor family of cell surface proteins. The human CD36 gene encodes a single chain 472 amino acid residue protein containing both an N- and a C-terminal cytoplasmic tail and an extracellular loop.CD36 is found on platelets, erythrocytes, monocytes, differentiated adipocytes, mammary epithelial cells, spleen cells and some skin microdermal endothelial cells. CD36 is a multiligand pattern recognition receptor that interacts with a large number of structurally dissimilar ligands, including long chain fatty acid (LCFA), advanced glycation end products (AGE), thrombospondin-1, oxidized low-density lipoproteins (oxLDLs), high density lipoprotein (HDL), phosphatidylserine, apoptotic cells, beta-amyloid fibrils (fAβ), collagens I and IV, and Plasmodium falciparum infected erythrocytes. CD36 is required for the anti-angiogenic effects of thrombospondin1 In the corneal neovascularization assay. On binding a ligand the protein and ligand are internalized. This internalization is independent of macropinocytosis and occurs by an actin dependent mechanism requiring the activation Src-family kinases, JNK and Rho-family GTPases. CD36 ligands have also been shown to promote sterile inflammation through assembly of a Toll-like receptor 4 and 6 heterodimer.

 

前沿进展

 
 
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CD36靶点信息
英文全称:Platelet glycoprotein 4
中文全称:血小板糖蛋白4
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:0详情
最高研发阶段:临床前
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