ActiveMax® Human TNF-alpha Protein, Tag Free, low endotoxin (active trimer) (MALS verified)

分子别名（Synonym）

DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα

表达区间及表达系统（Source）

ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (TNA-H4211) is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # P01375-1).

Predicted N-terminus: Val 77

蛋白结构（Molecular Characterization）

This protein carries no "tag".

The protein has a calculated MW of 17.4 kDa. The protein migrates as 17 kDa under reducing (R) condition (SDS-PAGE).

内毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70^°C for 3 months under sterile conditions after reconstitution.