背景
由SARS-CoV-2造成的大流行是国际关注的突发公共卫生事件。目前，大多数国家的疫情还没有达到高峰，预计未来几周和几个月的病例和死亡人数还将持续上升。准确诊断COVID-19对确保感染患者得到及时救治，对无症状感染者进行有效隔离，限制病毒传播至关重要。目前广泛应用的病原学检测基于对收集的鼻咽拭子样本进行实时PCR核酸检测(RT-PCR)但是在实际应用中发现大量的假阴性结果，COVID-19临床和流行病学疑似病例RT-PCR阳性检出率约为50%。
血清学检测基于寻找针对病原菌感染产生的抗体来确定人们是否接触过某种特定的病原体。血清学检测相对于RT-PCR有以下优势：首先，血清学检测的检测样本是人类抗体，比病毒RNA稳定得多，在采集、运输、储存和检测过程中稳定性高于RT-qPCR样本。其次，抗体通常均匀分布在血液中，容易采集样本且样本的差异性低于鼻咽部采集的病毒RNA样本，第三，血液中的病毒特异性抗体可以在感染后持续数周或数月，因此血清学检测可以检测人体是否曾受到过病毒感染。为了提高临床诊断的准确性，更好地确定包括无症感染者和康复患者在内的COVID-19病例的数量，中国疾病预防控制中心已在全国多地启动COVID-19血清流行病学抽样调查，国际上也在广泛开展血清抗体检测，对COVID-19血清学检测的需求正在迅速增加。
截至5月17日，已有 12种COVID-19血清学检测试剂盒获得美国FDA的紧急使用授权，包括胶体金法、化学发光法和ELISA法。这些商业化试剂盒中使用重组表达的SARS-CoV-2的抗原来检测SARS-CoV-2感染患者血清中的抗体，可提高血清学检测试剂盒的灵敏度和特异性。
ACROBiosystems开发了专门针对血清学检测试剂盒设计和优化的SARS-CoV-2抗原产品，包括S1、RBD和N蛋白，S1、RBD蛋白基于HEK293细胞生产， N蛋白基于HEK293细胞或大肠杆菌细胞生产。抗原通过多种标签表达，包括His、hFc、mFc和His&Avi，此外，ACROBiosystems还开发了5种 RBD突变型蛋白产品，以满足血清学检测发展的需要。
ACROBiosystems的S1、RBD和N蛋白组分可根据客户需求进行定制化生产，适合于COVID-19血清学检测胶体金法、化学发光法和ELISA法，有助于SARS-CoV-2（COVID-19）检测试剂盒的开发生产。ACROBiosystems提供的S1、RBD和N蛋白活性已经过验证，并可大量供应。
活性数据
经ELISA验证，N蛋白结合抗单克隆抗体的灵敏度为0.02 ng/mL
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Fig.1 Immobilized SARS-CoV-2 Nucleocapsid protein, His Tag (Cat. No. NUN-C51H9) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 NP Antibody, Rabbit MAb (CLN27) with a linear range of 0.02-0.3 ng/mL.
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经ELISA验证，S1蛋白与ACE2蛋白结合的灵敏度为0.2 ng/mL
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Fig.2 Immobilized SARS-CoV-2 S1 protein, His Tag (Cat. No. S1N-C52H4) at 2 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.2-6 ng/mL.
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经ELISA验证，RBD蛋白与ACE2蛋白结合的灵敏度为8 ng/mL
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Fig.3 Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) with a linear range of 8-125 ng/mL.
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产品列表
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点击提议
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Molecule
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Cat. No.
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Product Description
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S1 protein
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S1N-C52H3
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SARS-CoV-2 (COVID-19) S1 protein, His Tag
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S1N-C82E8
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Biotinylated SARS-CoV-2 (COVID-19) S1 protein, His,Avitag™ (MALS verified)
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S1N-S52H5
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SARS S1 protein, His Tag (MALS verified)
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S1N-C52H4
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SARS-CoV-2 (COVID-19) S1 protein, His Tag (MALS verified)
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S1N-C5255
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SARS-CoV-2 (COVID-19) S1 protein, Fc Tag
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S1N-C5257
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SARS-CoV-2 (COVID-19) S1 protein, Mouse IgG2a Fc Tag
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S protein RBD
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SPD-C82E9
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Biotinylated SARS-CoV-2 (COVID-19) S protein RBD, His,Avitag™ (MALS verified)
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SPD-C5255
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SARS-CoV-2 (COVID-19) S protein RBD, Fc Tag (MALS verified)
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SPD-S52H6
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SARS S protein RBD, His Tag (MALS verified)
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SPD-C52H3
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SARS-CoV-2 (COVID-19) S protein RBD, His Tag (MALS verified)
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SPD-C5259
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SARS-CoV-2 (COVID-19) S protein RBD, Mouse IgG2a Fc Tag
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SPD-S52H4
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SARS-CoV-2 (COVID-19) S protein RBD (V367F), His Tag
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SPD-S52H5
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SARS-CoV-2 (COVID-19) S protein RBD (N354D), His Tag
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SPD-S52H7
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SARS-CoV-2 (COVID-19) S protein RBD (W436R), His Tag
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SPD-S52H8
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SARS-CoV-2 (COVID-19) S protein RBD (R408I), His Tag
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SPD-S52H3
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SARS-CoV-2 (COVID-19) S protein RBD (N354D, D364Y), His Tag
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Nucleocapsid protein
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NUN-C51H9
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SARS-CoV-2 (COVID-19) Nucleocapsid protein, His Tag
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NUN-C5227
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SARS-CoV-2 (COVID-19) Nucleocapsid protein, His Tag
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NUN-C81Q6
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Biotinylated SARS-CoV-2 (COVID-19) Nucleocapsid protein, His,Avitag™
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