|CD3-H82E3||Human||His Tag & Avi Tag|
Fig.1 The purity of Human CD73, His Tag (HPLC-verified) (Cat. No. CD3-H52H7) was greater than 90% as determined by SEC-HPLC.
Fig.2 Loaded Anti-CD73 MAb (Human IgG1) on AHC Biosensor, can bind Human CD73, His Tag (Cat. No. CD3-H52H7) with an affinity constant of 0.179 nM as determined in BLI assay (ForteBio Octet Red96e).
Fig.3 Anti-CD73 antibody (Human IgG1) captured on CM5 chip via anti-human IgG Fc antibodies surface can bind Human CD73, His Tag, HEK293 (Cat. No. CD3-H52H7) with an affinity constant of 0.164 nM as determined in SPR assay (Biacore 8K).
Fig.4 Immobilized Human CD73, His Tag (HPLC-verified) (Cat. No. CD3-H52H7) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD73 Antibody, Human IgG1 with a linear range of 0.15-5 ng/mL.