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>产品 > 荧光标记蛋白

荧光标记蛋白

近年来，流式细胞术被广泛应用于生物药研发和生产的各个环节。荧光标记蛋白作为流式检测的首选试剂，因其预先标记了荧光素基团，可通过一步染色完成检测，既简化了检测流程、节省了检测时间，又避免了因使用二抗而产生的非特异背景。然而，高质量荧光标记蛋白的生产面临着诸多难题，如标记后蛋白活性难以维持、产品的批间一致性难以控制等，目前市场上少有高质量荧光标记蛋白可供购买。

ACROBiosystems作为专注于生物药研发领域的蛋白供应商，利用专业的蛋白研发平台、优化设计的化学标记平台及荧光定点标记平台、稳定株开发平台和流式细胞分析平台，开发了一系列高批间一致性的FITC标记和PE标记等荧光标记蛋白，适用于基于流式细胞术的分析试验，如CAR阳性表达率的检测和流式细胞术筛选抗体候选药物等。

产品特色
  1. 标记后蛋白活性经流式细胞术验证
  2. 可提供配套流式检测protocol供参考
  3. 标记前后活性保持一致
  4. 高批间一致性
应用案例
流式细胞术检测Anti-CD19 CAR阳性表达率
FACS Analysis of Anti-CD19 CAR Expression
293 cells were transfected with anti-CD19-scFv and RFP tag. 2e5 of the cells were stained with FITC-Labeled Human CD19 (20-291), Fc Tag (Cat. No. CD9-HF251, 10 μg/ml) and FITC-labeled protein control. Non-transfected 293 cells and FITC-labeled protein control were used as negative control. RFP was used to evaluate CAR (anti-CD19-scFv) expression and FITC was used to evaluate the binding activity of FITC-Labeled Human CD19 (20-291), Fc Tag (Cat. No. CD9-HF251) (QC tested).
Protocol
基于流式细胞术的配体结合试验
Flow Cytometry assay shows that PE-Labeled Human PD-1, Fc Tag, His Tag (recommended for neutralizing assay) (Cat. No. PD1-HP2F2) can bind to 293T cells overexpressing human PD-L1. 1μl stock solution per million cells (QC tested).
Protocol
基于流式细胞术的中和抗体筛选试验
FACS analysis shows that the binding of PE-Labeled Human PD-1, Fc Tag, His Tag (recommended for neutralizing assay) (Cat.No.PD1-HP2F2) to 293T overexpressing PD-L1 was inhibited by increasing concentration of neutralizing antihuman PD-L1 antibody. The IC50 is 0.29 μg/ml (QC tested).
Protocol
质控
高批间一致性
Binding activity of three different lots of FITC-labeled Human MSLN, Fc Tag was evaluated in the above FACS analysis against Anti-MSLN CAR-293 cells. The result shows very high batch-to-batch consistency.
Binding activity of three different lots of FITC-labeled Human MSLN, Fc Tag was evaluated in the above ELISA analysis against Anti-MSLN Antibody. The result shows very high batch-to-batch consistency.
标记前后活性保持一致
Binding activity of the Human MSLN, Fc Tag before and after FITC labeling was evaluated in the above ELISA analysis. The result shows that FITC-Labeled and Unconjugated Human MSLN, Fc Tag have almost the same level of binding activity.
稳定性好
Binding activity of FITC-labeled Human MSLN, Fc Tag was evaluated in the above FACS analysis against Anti-MSLN CAR-293 cells. The result shows that the FITC-labeled Human MSLN is stable at 37℃ for 24 hours without performance reduction.
Binding activity of FITC-labeled Human MSLN, Fc Tag was evaluated in the above FACS analysis against Anti-MSLN CAR-293 cells. The result shows that the FITC-labeled Human MSLN is stable within three freeze-thaw cycles without performance reduction.
产品列表
PE Labeled Protein
Molecule Cat. No. Species Product Description Host Structure
BCMA BCA-HP2H2 Human PE-Labeled Human BCMA / TNFRSF17 Protein, His Tag HEK293
CD19 CD9-HP2H3 Human PE-Labeled Human CD19 (20-291) Protein, His Tag HEK293
LAG-3 LA3-HP2H3 Human PE-Labeled Human LAG-3 / CD223 Protein, His Tag (recommended for neutralizing assay) HEK293
Mesothelin MSN-HP223 Human PE-Labeled Human Mesothelin / MSLN (296-580) Protein, His Tag HEK293
PD-1 PD1-HP2F2 Human PE-Labeled Human PD-1 / PDCD1 Protein, Fc Tag, His Tag (recommended for neutralizing assay) HEK293
Siglec-10 SI0-HP2E5 Human PE-Labeled Human Siglec-10 Protein, His Tag (recommended for neutralizing assay) HEK293
Siglec-3 CD3-HP2E3 Human PE-Labeled Human Siglec-3 / CD33 Protein, His Avitag™ HEK293
SIRP alpha SIA-HP252 Human PE-Labeled Human SIRP alpha / CD172a Protein, Fc Tag (recommended for neutralizing assay) HEK293
SLAMF7 SL7-HP2H3 Human PE-Labeled Human SLAMF7 / CRACC / CD319 Protein, His Tag HEK293
FITC Labeled Protein
Molecule Cat. No. Species Product Description Host Structure
B7-H3 (4Ig) B7B-HF2E7 Human FITC-Labeled Human B7-H3 (4Ig) / B7-H3b Protein, His Tag (MALS verified) HEK293
BAFF BAF-HF268 Human FITC-Labeled Human BAFF / TNFSF13B / CD257 Protein, Fc Tag HEK293
BCMA BCA-HF254 Human FITC-Labeled Human BCMA / TNFRSF17 Protein, Fc Tag DMF Filed HEK293
BCA-HF2H1 Human FITC-Labeled Human BCMA / TNFRSF17 Protein, His Tag DMF Filed HEK293
CD19 CD9-HF251 Human FITC-Labeled Human CD19 (20-291) Protein, Fc Tag DMF Filed HEK293
CD9-HF2H2 Human FITC-Labeled Human CD19 (20-291) Protein, His Tag DMF Filed HEK293
CD27 Ligand CDD-HF2D4 Human FITC-Labeled Human CD27 Ligand / CD70 Protein, His,Flag Tag active trimer (MALS verified) HEK293
CD30 CD0-HF250 Human FITC-Labeled Human CD30 / TNFRSF8 Protein, Fc Tag HEK293
CD0-HF2H3 Human FITC-Labeled Human CD30 / TNFRSF8 Protein, His Tag HEK293
CD38 CD8-HF255 Human FITC-Labeled Human CD38 Protein, Fc Tag HEK293
CD8-HF2H5 Human FITC-Labeled Human CD38 Protein, His Tag HEK293
CD4 CD4-HF255 Human FITC-Labeled Human CD4 Protein, Fc Tag HEK293
CD4-HF2H7 Human FITC-Labeled Human CD4 Protein, His Tag HEK293
CD47 CD7-HF2H3 Human FITC-Labeled Human CD47 Protein, His Tag HEK293
CD5 CD5-H52H2 Human FITC-Labeled Human CD5 Protein, His Tag (MALS verified) HEK293
CD7 CD7-HF257 Human FITC-Labeled Human CD7 Protein, Llama IgG2b Fc Tag HEK293
CD7-HF2H6 Human FITC-Labeled Human CD7 Protein, His Tag HEK293
CD72 CD2-HF258 Human FITC-Labeled Human CD72 Protein, Fc Tag HEK293
CD8 alpha & beta CDA-HF2W4 Human FITC-Labeled Human CD8 alpha&beta (CD8A&CD8B) Heterodimer Protein, His Tag&Tag Free HEK293
CEACAM-5 CE5-HF254 Human FITC-Labeled Human CEACAM-5 / CD66e Protein, His Tag (MALS verified) HEK293
CLEC12A CLA-HF247 Human FITC-Labeled Human CLEC12A / MICL / CLL-1 Protein, His Tag HEK293
DLL3 DL3-HF253 Human FITC-Labeled Human DLL3 Protein, Fc Tag HEK293
DL3-HF2H4 Human FITC-Labeled Human DLL3 Protein, His Tag HEK293
DNP DNP-FM1 Mouse FITC-Labeled Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control HEK293
DNP-FM2 Human FITC-Labeled Monoclonal Anti-DNP antibody, Human IgG1 Isotype Control HEK293
EGF R EGR-HF2H5 Human FITC-Labeled Human EGF R Protein, His Tag HEK293
EGFRvIII EGI-HF2H7 Human FITC-Labeled Human EGFRvIII Protein, His Tag HEK293
EMMPRIN CD7-HF222 Human FITC-Labeled Human EMMPRIN / CD147 Protein, His Tag HEK293
EpCAM EPM-HF225 Human FITC-Labeled Human EpCAM / TROP1 Protein, His Tag HEK293
EPM-HF255 Human FITC-Labeled Human EpCAM / TROP1 Protein, Fc Tag HEK293
ErbB3 ER3-HF2H5 Human FITC-Labeled Human ErbB3 / Her3 Protein, His Tag HEK293
FAP FAP-HF243 Human FITC-Labeled Human FAP Protein, His Tag (MALS verified) HEK293
FAP-HF263 Human FITC-Labeled Human FAP Protein, Fc Tag HEK293
FMC63 FM3-FY45 Mouse FITC-Labeled Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Y45) Hybridoma
FOLR1 FO1-HF2H8 Human FITC-Labeled Human FOLR1 Protein, His Tag (MALS verified) HEK293
Glypican 3 GP3-HF258 Human FITC-Labeled Human Glypican 3 / GPC3 Protein, Fc Tag HEK293
GP3-HF2H1 Human FITC-Labeled Human Glypican 3 / GPC3 Protein, His Tag HEK293
GUCY2C GUC-HF2H8 Human FITC-Labeled Human GUCY2C / Guanylyl cyclase C Protein, His Tag HEK293
Her2 HE2-HF224 Human FITC-Labeled Human Her2 / ErbB2 Protein, His Tag HEK293
IL-13 R alpha 2 IL2-HF2H3 Human FITC-Labeled Human IL-13 R alpha 2 Protein, His Tag HEK293
IL-3 R alpha ILA-HF2H9 Human FITC-Labeled Human IL-3 R alpha / CD123 Protein, His Tag HEK293
Mesothelin MSN-HF223 Human FITC-Labeled Human Mesothelin / MSLN (296-580) Protein, His Tag HEK293
MSN-HF253 Human FITC-Labeled Human Mesothelin / MSLN (296-580) Protein, Fc Tag HEK293
MSN-HF25x Human FITC-Labeled Human Mesothelin / MSLN (296-580) Protein, Fc Tag HEK293
Nectin-4 NE4-HF2H3 Human FITC-Labeled Human Nectin-4 Protein, His Tag HEK293
NKG2D NKD-HF224 Human FITC-Labeled Human NKG2D / CD314 Protein, Fc Tag HEK293
PSMA PSA-HF244 Human FITC-Labeled Human PSMA / FOLH1 Protein, His Tag HEK293
Rituximab RIB-FY35c Mouse FITC-Labeled Anti-Rituximab Antibodies, Mouse IgG1 Hybridoma
ROR1 RO1-HF253 Human FITC-Labeled Human ROR1 Protein, Fc Tag HEK293
RO1-HF258 Human FITC-Labeled Human ROR1 Protein, His Tag HEK293
Siglec-2 SI2-HF2H6 Human FITC-Labeled Human Siglec-2 / CD22 Protein, His Tag HEK293
Siglec-3 CD3-HF224 Human FITC-Labeled Human Siglec-3 / CD33 Protein, His Tag HEK293
CD3-HF256 Human FITC-Labeled Human Siglec-3 / CD33 Protein, Fc Tag HEK293
SLAMF7 SL7-HF2H7 Human FITC-Labeled Human SLAMF7 / CRACC / CD319 Protein, His Tag HEK293
Syndecan-1 SD1-HF228 Human FITC-Labeled Human Syndecan-1 Protein, His Tag HEK293
VEGF R2 VE2-HF254 Human FITC-Labeled Human VEGF R2 / KDR Protein, His Tag HEK293
产品定制

为满足生物药研发和生产领域客户的需求，ACROBiosystems正在积极开发高质量的荧光标记蛋白产品。如果您愿意分享您在这个领域的想法和需求，我们将非常感谢。如果您有任何建议或定制要求，请随时与我们联系。

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