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新冠疫苗开发与评价的核心试剂

背景
由新冠病毒(SARS-CoV-2)引起的COVID-19大流行是一个世纪以来人类面临的最严峻挑战,一种安全有效的疫苗对于控制疫情和防止再次暴发具有重要意义。目前,针对COVID-19的疫苗研发进展飞快,病毒灭活疫苗、基因工程重组疫苗、病毒载体类疫苗、核酸类疫苗(质粒DNA、mRNA等)等多条技术路线齐头并进,其中已经有多款新冠候选疫苗进入3期临床试验阶段,其安全性、免疫原性、有效性等问题备受关注。
ACROBiosystems基于在重组蛋白领域多年的深耕和技术积累,针对新冠病毒疫苗研究领域开发了包括经MALS和负染电镜验证的三聚体S蛋白在内的多种重组蛋白、检测试剂盒等核心试剂,可用于检测IgG/M抗体滴度、中和抗体滴度、抗原滴度等,助力新冠病毒疫苗开发与评价。
应用
应用场景1:IgG抗体滴度检测
推荐产品:

货号:SPN-C52H9 名称:SARS-CoV-2 S protein, His Tag, Super stable trimer (MALS & NS-EM verified)

蛋白纯度、分子量和聚体形式经SDS-PAGE, SEC-MALS 和负染电镜技术严格验证

The purity of SARS-CoV-2 S protein, His Tag, Super stable trimer (Cat. No. SPN-C52H9) was more than 90% verified by SDS-PAGE under reducing (R) condition. The molecular weight was around 550-660 kDa confirmed by SEC-MALS. The particles are similar in size and appearance to SARS-CoV-2 trimers reported in published literature verified by negative stain electron micrography.

Protocol

ELISA验证S蛋白与新冠病毒抗体结合活性强

Immobilized Anti-SARS-CoV-2 Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein, His Tag, Super stable trimer (Cat. No. SPN-C52H9) with a linear range of 0.3-10 ng/mL

Protocol

货号:SPD-C82E9 名称:Biotinylated SARS-CoV-2 (COVID-19) S protein RBD, His,Avitag™ (MALS verified)

ELISA验证S RBD蛋白与新冠病毒抗体结合活性强
Immobilized Biotinylated SARS-CoV-2 S protein RBD, His,Avitag (Cat. No. SPD-C82E9) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Monoclonal Anti-SARS-CoV-S protein RBD Antibody, Human IgG1 with a linear range of 0.1-4 ng/mL
应用案例:
辉瑞和BioNTech新冠疫苗1/2期临床研究在检测与RBD结合的IgG抗体水平时,使用了ACROBiosystems生产的生物素化RBD蛋白(货号:SPD-C82E9),通过将此蛋白与链霉亲和素包被的微粒结合,来捕获血液样本中结合RBD的IgG抗体,获得了满意的实验结果。[1]

Phase 1/2 Study to Describe the Safety and Immunogenicity of a COVID-19 RNA Vaccine Candidate (BNT162b1) in Adults 18 to 55 Years of Age: Interim Report. medRxiv preprint. doi: https://www.medrxiv.org/content/10.1101/2020.06.30.20142570v1

货号:TAS-K002 名称:Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kit (Spike protein RBD)

S RBD蛋白抗体滴度检测试剂盒采用间接ELISA法检测血清中新冠病毒抗体IgG滴度,检测灵敏度为24 ng/mL
Immobilized SARS-CoV-2 S protein RBD at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-2 Antibody, Human IgG1 in 1:50 human serum. Detection was performed using HRP-Conjugated Anti-human IgG antibody with sensitivity of 24 ng/mL
应用场景2:中和抗体滴度检测
推荐产品:

货号:SPD-C52H3 名称:SARS-CoV-2 (COVID-19) S protein RBD, His Tag (MALS verified)

ELISA验证,S RBD蛋白与RBD中和抗体结合活性强

Immobilized SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 RBD Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35) with a linear range of 0.1-3 ng/mL

Protocol

ELISA验证,S RBD蛋白与ACE2蛋白结合活性强

Immobilized SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.2-3 ng/mL

Protocol

货号:TAS-K003 名称:Anti-SARS-CoV-2 Neutralizing Antibody Titer Serologic Assay kit

中和抗体滴度检测试剂盒可检测血清中的中和抗体
Neutralizing titer of Anti-SARS-CoV-2 Neutralizing Antibody, Human IgG1 (Cat.No.SAD-S35) measured by Anti-SARS-CoV-2 neutralizing antibody titer serologic assay kit (Cat. No. TAS-K003).
应用场景3:抗原滴度检测
推荐产品:

货号:TAS-K011 名称:SARS-CoV-2 Spike Protein Titer Assay Kit

S抗原滴度检测试剂盒检测S蛋白的灵敏度为24 pg/mL
Immobilized Anti-SARS-CoV-2 Spike S1 Antibody at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike Protein. And then add Biotinylated Anti-SARS-CoV-2 Spike S1 Antibody at 1ug/ml. Detection was performed using HRP-conjugated streptavidin with sensitivity of 24 pg/mL
应用场景4:抗体亚型检测

ACROBiosystems还开发了抗体亚型检测系列试剂盒,可用于新冠病毒抗体IgG1、IgG2、IgG3和IgG4四种抗体亚型的检测;同时,可用于临床前检测不同种属抗体的试剂盒即将上线,敬请期待!

货号 产品名称
TAS-K014 Anti-SARS-CoV-2 IgG1 Titer Serologic Assay Kit (S Protein RBD)
TAS-K015 Anti-SARS-CoV-2 IgG2 Titer Serologic Assay Kit (S Protein RBD)
TAS-K016 Anti-SARS-CoV-2 IgG3 Titer Serologic Assay Kit (S Protein RBD)
TAS-K017 Anti-SARS-CoV-2 IgG4 Titer Serologic Assay Kit (S Protein RBD)
产品列表
重组蛋白类
分子 货号 产品名称
ACE2 protein AC2-H52H8 Human ACE2 / ACEH Protein, His Tag (MALS verified)
AC2-H82E6 Biotinylated Human ACE2 / ACEH Protein, His,Avitag™ (MALS verified)
AC2-H5257 Human ACE2 / ACEH Protein, Fc Tag (MALS verified)
AC2-H82F9 Biotinylated Human ACE2 / ACEH Protein, Fc,Avitag™
S trimer protein SPN-C52H9 SARS-CoV-2 S protein, His Tag, Super stable trimer (MALS & NS-EM verified)
SPN-C82E9 Biotinylated SARS-CoV-2 S protein, His,Avitag™, Super stable trimer (MALS verified)
SPN-C52H3 SARS-CoV-2 S protein (D614G), His Tag, Super stable trimer (MALS verified)
SPN-C82E3 Biotinylated SARS-CoV-2 S protein (D614G), His,Avitag™, Super stable trimer (MALS verified)
S protein RBD SPD-C52H3 SARS-CoV-2 (COVID-19) S protein RBD, His Tag (MALS verified)
SPD-C82E9 Biotinylated SARS-CoV-2 (COVID-19) S protein RBD, His,Avitag™ (MALS verified)
SPD-C5259 SARS-CoV-2 (COVID-19) S protein RBD, Mouse IgG2a Fc Tag(MALS verified)
SPD-C82Aa Biotinylated SARS-CoV-2 (COVID-19) S protein RBD, Mouse IgG1 Fc,Avitag™(MALS verified)
S1 protein S1N-C52H4 SARS-CoV-2 (COVID-19) S1 protein, His Tag (MALS verified)
S1N-C82E8 Biotinylated SARS-CoV-2 (COVID-19) S1 protein, His,Avitag™ (MALS verified)
S1N-C5256 SARS-CoV-2 (COVID-19) S1 protein (D614G), His Tag
S1N-C82E3 Biotinylated SARS-CoV-2 (COVID-19) S1 protein (D614G), His,Avitag™
S1 protein NTD S1D-C52H6 SARS-CoV-2 (COVID-19) S1 protein NTD, His Tag
S1 protein CTD S1D-C52H3 SARS-CoV-2 (COVID-19) S1 protein CTD, His Tag (MALS-verified)
S2 protein S2N-C52H5 SARS-CoV-2 (COVID-19) S2 protein, His Tag
Nucleocapsid protein NUN-C5227 SARS-CoV-2 (COVID-19) Nucleocapsid protein, His Tag
NUN-C82E8 Biotinylated SARS-CoV-2 (COVID-19) Nucleocapsid protein, His,Avitag™
试剂盒类
类别 货号 产品名称
中和抗体滴度检测试剂盒 TAS-K003 Anti-SARS-CoV-2 neutralizing antibody titer serologic assay kit
抗体滴度检测试剂盒 TAS-K007 Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kit (S protein trimer)
TAS-K001 Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kit (S protein S1)
TAS-K002 Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kit (S protein RBD)
TAS-K008 Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kit (Nucleocapsid Protein)
TAS-K009 Anti-SARS-CoV-2 Total Antibody Titer Serologic Assay kit(antigen sandwich)
TAS-K012 Anti-SARS-CoV-2 Antibody IgM Serologic Assay kit (S protein S1)
TAS-K013 Anti-SARS-CoV-2 Antibody IgM Serologic Assay kit (S RBD protein)
抗原检测试剂盒 TAS-K010 SARS-CoV-2 Nucleocapsid Protein Titer Assay Kit
TAS-K011 SARS-CoV-2 Spike Protein Titer Assay Kit
抗体亚型检测试剂盒 TAS-K014 Anti-SARS-CoV-2 IgG1 Titer Serologic Assay Kit (S Protein RBD)
TAS-K015 Anti-SARS-CoV-2 IgG2 Titer Serologic Assay Kit (S Protein RBD)
TAS-K016 Anti-SARS-CoV-2 IgG3 Titer Serologic Assay Kit (S Protein RBD)
TAS-K017 Anti-SARS-CoV-2 IgG4 Titer Serologic Assay Kit (S Protein RBD)
抗体类
分子 货号 产品名称
S protein RBD SAD-S35 Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Human IgG1 (AS35)
SPD-M128 Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Mouse/Human Chimeric mAb, Human IgG1 (AM128)
S1 protein S1N-M122 Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM122)
S1N-M130 Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM130)
参考文献:
[1] Mulligan et al., (2020). Phase 1/2 Study to Describe the Safety and Immunogenicity of a COVID-19 RNA Vaccine Candidate (BNT162b1) in Adults 18 to 55 Years of Age: Interim Report. medRxiv, doi: https://doi.org/10.1101/2020.06.30.20142570 )

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