图1 利用磁珠进行抗体筛选的过程
分子 | 货号 | 产品描述 | 种属 | 订购/预购 |
---|---|---|---|---|
S1 protein | MBS-K001 | SARS-CoV-2 Spike S1 protein-coupled magnetic beads | SARS-CoV-2 | |
S protein RBD | MBS-K002 | SARS-CoV-2 Spike protein RBD-coupled magnetic beads | SARS-Cov-2 | |
CD3 | MBS-K003 | CD3 epsilon&CD3 delta Heterodimer protein-coupled magnetic beads | Human | |
BCMA | MBS-K004 | BCMA-coupled magnetic beads | Human | |
CD19 | MBS-K005 | CD19-coupled magnetic beads | Human | |
HER2 | MBS-K006 | HER2-coupled magnetic beads | Human | |
ACE2 | MBS-K013 | Human ACE2-coupled magnetic beads | SARS-CoV-2 | |
S protein RBD | MBS-K014 | Anti-SARS-CoV-2 Spike RBD Antibody-coupled magnetic beads | SARS-CoV-2 | |
S protein | MBS-K015 | SARS-CoV-2 Spike S trimer protein-coupled magnetic beads | SARS-CoV-2 | |
MBS-K016 | SARS-CoV-2 Spike S trimer (D614G) protein-coupled magnetic beads | SARS-CoV-2 | ||
Nucleocapsid protein | MBS-K017 | SARS-CoV-2 Nucleocapsid protein-coupled magnetic beads | SARS-CoV-2 | |
PSMA | MBP-K007 | PSMA-coupled magnetic beads | Human | |
CD33 | MBC-K008 | CD33-coupled magnetic beads | Human | |
ROR1 | MBR-K009 | ROR1-coupled magnetic beads | Human | |
CD38 | MBC-K010 | CD38-coupled magnetic beads | Human | |
EpCAM | MBE-K011 | EpCAM-coupled magnetic beads | Human | |
EGFR | MBE-K012 | EGFR-coupled magnetic beads | Human |
ACROBiosystems对比多种磁珠,精选出粒径均匀,形状规整,具有超顺磁性的磁珠,得到了最低的非特异性结合。
图2 不同磁珠形态对比(A图为ACROBiosystems精选磁珠)
链霉亲和素(SA)和生物素之间亲和力极强,生物素化蛋白预偶联不可逆。预偶联蛋白磁珠表面包被了饱和浓度的生物素标记抗原,结合能力强,可有效提升捕获效率。
图3 RBD偶联磁珠结合抗体量曲线
图4 RBD偶联磁珠和ACE2蛋白的结合曲线
Protocol
图5 BCMA偶联磁珠和抗BCMA抗体的结合曲线
ACROBiosystems特别设计的预偶联蛋白磁珠为冻干状态,可长期稳定储存,多次冻融不影响结构和性能。重构后即可使用,无需活化,操作简便,可大大提高您的实验效率,节约时间和成本。
图6. ACRO磁珠在不同状态下的电镜图片 (Cat. No. SMB-B01),结果表明磁珠在3次冻融后结构稳定。
图7. 多次冻融后SARS-CoV-2 S RBD蛋白偶联磁珠(货号:MBS-K002)与S1抗体结合曲线与冻融前无显著性差异。
图8. 多次冻融后BCMA蛋白偶联磁珠(货号:MBS-K004)与BCMA抗体结合曲线与冻融前无显著性差异。